Protein

Question 1

Proteins that are Found only in animals.

Answer 1

Fibrous protein

Question 2

Don’t serve structural functions. They can act as transporters like hemoglobin and are often enzymes.

Answer 2

Globular proteins

Question 3

Proteins that are Generally insoluble in water.

Answer 3

Fibrous protein

Question 4

Proteins that are usually water-soluble.

Answer 4

Globular protein

Question 5

is a fibrous protein found in skin and bone.

Answer 5

Collagen

Question 6

are proteins that facilitate chemical reactions under mild conditions.

Answer 6

Enzymes

Question 7

is a transport protein for oxygen.

Answer 7

Hemoglobin

Question 8

are proteins that fight foreign substances.

Answer 8

Antibodies

Question 9

stores iron in the liver.

Answer 9

Ferritin

Question 10

most fundamental level that all proteins have

Answer 10

Primary level

Question 11

most specific level that only some proteins have

Answer 11

Quarternary level

Question 12

is required for protein synthesis.

Answer 12

Energy

Question 13

what remains of each amino acid is called

Answer 13

Residue

Question 14

When two or more amino acids combine,
a molecule of water is

Answer 14

Removed

Question 15

ultimately controls the amino acid sequence, with mRNA serving as the template.

Answer 15

DNA/deoxyribonucleic acid

Question 16

allows for hydrogen bonding between residues.

Answer 16

Backbone

Question 17

was one of the first proteins to have its primary structure determined.

Answer 17

Bovine insulin

Question 18

refers to the folding of the polypeptide chain due to hydrogen bonds between peptide bonds.

Answer 18

Secondary structure

Question 19

is a secondary structure formed by hydrogen bonding between the amino acids in a polypeptide chain.

Answer 19

a-helix

Question 20

the protein found in hair, nails, and fur

Answer 20

Keratin

Question 21

creates bends or “kinks” that inhibit the formation of a regular pattern of hydrogen bonds.

Answer 21

Proline

Question 22

Strands align in the same direction

Answer 22

Parallel structure

Question 23

Strands align in opposite directions.

Answer 23

Antiparallel structure

Question 24

are typically four to five strands wide, but can be up to ten or more.

Answer 24

Sheets

Question 25

also known as a hairpin bend, is the simplest of these structures.

Answer 25

B-turn

Question 26

is a larger structure that resembles the Greek letter omega

Answer 26

Ω-loop

Question 27

The combination of primary, secondary structures, and side chain interactions

Answer 27

Tertiary structure

Question 28

repelled by water, but attracted to each other.

Answer 28

Hydrophobic

Question 29

Attract other polar side chains through dipole-dipole forces or hydrogen bonds.

Answer 29

Polar side chains

Question 30

Nonpolar side chains tend to cluster together, avoiding water.

Answer 30

Hydrophobic interactions

Question 31

Formed by interactions between multiple polypeptide chains

Answer 31

Quarternary structure

Question 32

Separates proteins based on net charge in an electric field

Answer 32

Electrophoresis

Question 33

Separates proteins based on net charge

Answer 33

Ion exchange chromatography

Question 34

One of the characteristics of proteins or enzymes in the blood is

Answer 34

Polymorphism

Question 35

The determination of blood type in a-b-o system, first begun in 1901, is based on

Answer 35

Antigen- antibody reactions

Question 36

An antigen on the surface of red blood cells

Answer 36

Rh factor

Question 37

Linear sequence of amino acids

Answer 37

Primary structure

Question 38

Screw-like / spiral structure

Answer 38

a-helix

Question 39

Combination of secondary structure

Answer 39

Tertiary structure

Question 40

Most of the proteins have this type of structure

Answer 40

Tertiary structure

Question 41

Composed of tertiary structure

Answer 41

Quaternary structure

Question 42

Determines the 3 dimensional configuration and properties

Answer 42

Primary structure

Question 43

Primary structure starts at the

Answer 43

N-terminal amino acid

Question 44

Primary structure stops or ends at the

Answer 44

C-terminal amino acids

Question 45

It limits the rotation of proteins

Answer 45

Peptide bond

Question 46

It gives the structure stability

Answer 46

Peptide bond

Question 47

Results from interactions between peptide bonds and derived from primary structure

Answer 47

Secondary structure

Question 48

Type of B-pleated sheet that the direction of the carboxyl end is in the same direction

Answer 48

Parallel beta pleated sheet

Question 49

The bond that produces the secondary structure

Answer 49

Hydrogen bond

Question 50

Typically 4-5 strands wide

Answer 50

B-pleated sheet

Question 51

Results from interactions between side chains

Answer 51

Tertiary structure

Question 52

The overall 3D conformation

Answer 52

Tertiary structure

Question 53

Results from interactions between subunits (tertiary structure)

Answer 53

Quaternary structure

Question 54

Primary oxygen carrying protein

Answer 54

Hemoglobin

Question 55

Migration of ions in an electrical fields

Answer 55

Electrophoresis

Question 56

Prevents diffusion of proteins out of the gel

Answer 56

Fixation

Question 57

Stains for lipids

Answer 57

- fat red 7b - oil red O - Sudan black B

Question 58

Stain for serum proteins in general

Answer 58

- amido black - coomassie brilliant blue G-250 - coomassie brilliant blue R-250 - ponceau S

Question 59

Stains for isoenzymes

Answer 59

Nitrotetrazolium blue

Question 60

Stains for DNA fragments

Answer 60

Ethidium bromide

Question 61

Stain for CSF proteins

Answer 61

Silver nitrate

Question 62

How many agarose film can a 100ml stain solution stain?

Answer 62

387 cm^2 / 60 in^2

Question 63

The term if leaching occurs

Answer 63

Faulty

Question 64

How many acetic acid is involved in washing?

Answer 64

5% acetic acid

Question 65

Measures absorbance of each fraction

Answer 65

Photometric optical system