ENZYMOLOGY

Question 1

How are enzymes measured?

Answer 1

based on their activity

Question 2

factors affecting enzymatic reactions

Answer 2

1. Coenzymes

2. Activators

Question 3

General activator of enzymes

Answer 3

Ca, K

Question 4

No. 1 enzyme activator

Answer 4

Ca

Question 5

CK and ALP activator

Answer 5

Mg

Question 6

LD activator

Answer 6

Zn

Question 7

Amylase activator

Answer 7

Cl

Question 8

measured in enzyme measurement

Answer 8

product + free enzyme

Question 9

all that needs dehydrogenase

Answer 9

oxidoreductase

Question 10

all kinases and transferase

Answer 10

Transferase

Question 11

esterase and peptidase

Answer 11

Hydrolase

Question 12

enzymes under esterase

Answer 12

ACP, ALP, Lipase, Cholinesterase

Question 13

enzyme under esterase

Answer 13

LAP

Question 14

Most important marker for obstructive jaundice

Answer 14

ALP

Question 15

secondary pancreatic marker

Answer 15

Trypsin

Question 16

most important Lyase

Answer 16

aldolase

Question 17

ALP Isoenzymes

Answer 17

Liver, bone, placental, intestinal

Question 18

marked increased in

Answer 18

Paget’s disease & Obstrctive jaundice

Question 19

what is ordered together with ALP to confirm an Obstructive jaundice

Answer 19

GGT

Question 20

MOST anodal ALP

Answer 20

Liver ALP

Question 21

least anodal ALP

Answer 21

intestinal ALP

Question 22

most heat stable ALP

Answer 22

Placental ALP

Question 23

most heat labile ALP

Answer 23

Bone ALP

Question 24

Heat fractionization test/ heat stability test

Answer 24

std. temp: 56’C
time: 10-15 mins
no reagent

Question 25

Chemical inhibition tests for ALP

Answer 25

1. Phenylalanine 2. 3M Urea 3. Levamisole

Question 26

in Chemical inhibition tests for ALP, inhibits placental ALP

Answer 26

Phenylalanine

Question 27

inhibits bone ALP

Answer 27

3M urea

Question 28

Inhibits Major ALP

Answer 28

bone & liver

Question 29

Caranoplacental ALP

Answer 29

1. Regan ALP | 2. Nagao ALP

Question 30

Most heat stable Caranoplacental ALP

Answer 30

Regan ALP

Question 31

inhibited by Leucine and increased in Adenocarcinoma

Answer 31

Nagao ALP

Question 32

methods for ALP

Answer 32

1. Bessy-Lowry Brock | 2. Bower's and comb (ALP & GGT)

Question 33

most specific method for ALP

Answer 33

Bessy-Lowry Brock

Question 34

significant marker for MI, lover dse, & skeletal muscle disease

Answer 34

Transferase

Question 35

Uses Karmen method (Aspartate)

Answer 35

Transferase

Question 36

major tissue source of ALT

Answer 36

Liver