Eukaryotic DNA Replication - General Points

Question 1

Why is eukaryotic DNA replication so much more complex?

Answer 1

1. Large genome
2. DNA is more highly structured and organised
3. DNA is linear
4. Must be coordinated with cell cycle
5. DNA must be evaluated at checkpoints

Question 2

How is eukaryotic DNA replication similar to prokaryotic DNA replication?

Answer 2

1. Both occur before nuclear division
2. dsDNA is the DNA involved
3. 5’ to 3’ direction
4. SSB protein stabilises unwound DNA
5. Primase makes primer
6. Both bidirectional

Question 3

What phase of the cell cycle does replication occur?

Answer 3

S phase

Question 4

How many origins of replication are there?

Answer 4

Multiple

Question 5

At what speed does replication occur?

Answer 5

DNA replication occurs slowly in eukaryotic cells.

Question 6

What size Okazaki fragments are created?

Answer 6

Small Okazaki fragments are made on the lagging strand.

Question 7

Does eukaryotic DNA replication require DNA gyrase?

Answer 7

No.