General Mechanisms of DNA Replication

Question 1

4 main steps of DNA replication

Answer 1

1. Replication Fork Formation
2. Primer binding
3. Elongation
4. Termination

Question 2

How many primers are used on the leading strand?

Answer 2

One primer

Question 3

How many primers are used on the lagging strand?

Answer 3

Multiple primers

Question 4

In what nature is the leading strand synthesised

Answer 4

Continuously in a 5’ to 3’ direction

Question 5

In what nature is the lagging strand synthesised?

Answer 5

Discontinuously in Okazaki fragments and in the antiparallel direction to leading strand.
(3’ <—— 5’)

Question 6

What base-pairing mechanism is used to form the new strand?

Answer 6

Watson-Crick Complementary base-pairing where A binds T with 2 hydrogen bonds and C binds G with three hydrogen bonds.

Question 7

How and in what direction are phosphodiester bonds made to form the sugar-phosphate backbone

Answer 7

In the 5’ to 3’ direction where the 3’ carbon of the sugar binds the 5’ phosphate of the next molecule via covalent bonding