Prokaryotic DNA Replication - Termination

Question 1

Where is DNA replication terminated (E. coli)

Answer 1

Ter sites

Question 2

How many Ter sites does E. coli contain?

Answer 2

6: TerA, TerB, TerC, TerD, TerE and TerF

Question 3

Step 1

Answer 3

The two replication forks eventually meet at the termination region and a terminator utilisation substance protein (Tus) binds Ter to form a Tus-Ter complex.

Question 4

Step 2

Answer 4

Ter F, B and C arrest the clockwise moving fork and TerE, D and A arrest the anticlockwise moving fork. A trap is formed os the forks cannot leave termination region.