Exam 3 review

Question 1

Transposons

Answer 1

DNA sequences that move from one location to the other

Question 2

Conjugation

Answer 2

Bacteria transfer plasmids to each other

Question 3

Transduction

Answer 3

Viral vector

Question 4

Transformation

Answer 4

Bacteria pick up free DNA

Question 5

Retrotransposons

Answer 5

use reverse transcription

Question 5

DNA transposons

Answer 5

insert themselves into target DNA

Question 6

Hydrogels lab - what were we doing, and why?

Answer 6

Creating hydrogels with metal ions to test metal’s antimicrobial activity

Question 7

Merozoite

Answer 7

Have cilia, rhoptries, cause malaria

Question 8

Oocyst

Answer 8

Thick cell walls, dormant stage of parasite, clear and round,

Question 9

Euglena

Answer 9

Have flagellum, pear shaped, chloroplasts

Question 10

Amoebozoa

Answer 10

Has pseudopodia, has ectoplasm, and contractile vacuole

Question 11

Paramecium

Answer 11

Move with cilia, have pellicle, oval shaped

Question 12

What are tropical diseases?

Answer 12

Diseases that typically occur in tropical climates, underfunded and underresearched but still common, can be dangerous/deadly

Question 13

Remember some fun facts about brain-eating amoebas.

Answer 13

Found in water, almost 100% fatal, very rare, eat brain chemicals

Question 14

Ascomycota

Answer 14

form sacs, asexual/sexual reproduction (yeast/penicillin)

Question 15

Basidiomycota

Answer 15

Fruiting body, sexual reproduction (mushroom)

Question 16

Aspergilloma

Answer 16

Ball of fungus in lungs

Question 17

Ringworm

Answer 17

Fungal infection causing ring pattern

Question 18

Pseudomembranous Thrush

Answer 18

characterised by extensive white pseudomembranes consisting of desquamated epithelial cells, fibrin, and fungal hyphae

Question 19

Erythematous Thrush

Answer 19

Caused by overuse of antibiotics

Question 20

Ascaris lumbricoides

Answer 20

Found in feces, affect GI tract and lungs

Question 21

Trichinella spiralis

Answer 21

Undercooked meat, can affect heart and brain, causes fever, abdominal pain, nausea/vomiting

Question 22

Hookworm

Answer 22

Intestinal, blood feeding, caused by poor hygeine

Question 23

Schistosoma

Answer 23

Snails carry eggs in freshwater, can be asymptomatic, can damage bladder, kidneys and liver

Question 24

Helical

Answer 24

Helix

Question 25

Icosahedral

Answer 25

pentagon, many sides

Question 26

Complex

Answer 26

Oval

Question 27

Lytic

Answer 27

Uses reproductive cycle of host cell to make viruses

Question 28

Lysogenic

Answer 28

Inserting viral genome into host genome

Question 29

Herpes simplex

Answer 29

parental capsid docks onto a host nuclear pore complex (NPC), genome translocates through the nuclear pore into the nucleoplasm, where it is transcribed and replicated

Question 30

Hookworm is transmitted through

Answer 30

Dirty water

Question 31

Trichinella spiralis is transmitted through

Answer 31

undercooked meat

Question 32

You repeat your previous experiment with a different phage stock solution. You use the same dilution, but you end up with plates that [unexpected results here - different per version]. Your negative control, which has bacteria but no phage, has formed a turbid lawn. Interpret your results and suggest a possible next step.

Answer 32

Natual variation (EG going from 17 to 2) or mismeasured broth, you could use a spectrophotometer to check concentration of solution

Question 33

MOMA Project

Answer 33

Streptococcus pneumonae-
Lancet shaped gram positive bacteria causing
pneumonia, (Chest pain, cough with pleghm,
fluid buildup in lungs)

Question 34

SARS-CoV-2 life cycle

Answer 34

Binds to ACE 2 receptor, releases RNA, translation, transcription, translocation, assembly, reproduction

Question 35

HIV Genes/protein processing

Answer 35

Using reverse transcriptase