Exam Review (The real one) Flashcards
(52 cards)
Know about common sense safety rules and the significance of microbiology (generally)
Dress appropriately.
Keep a tidy lab.
Know your lab safety symbols.
Handle chemical spills properly.
Know your safety equipment.
Major contributions of microbiologists in history
Vaccines, medicine, germ theory, gram staining, antibiotics
BSL 1
1- Low risk, not known to cause disease in healthy humans
BSL 2
2- Moderate risk, EG common cold
BSL 3
3- High risk, can cause serious or potentially deadly disease (TB, Plague)
BSL 4
4- Life threatening, possibly nontreatable (Ebola, smallpox)
Our Epidemic lab, what were we modeling?
Viral transmission in large populations
Bacteria
-lack of membrane-bound organelles,
-Unicellular
-small (usually microscopic)
-Reproduce by binary fission
Viruses
-Much smaller than bacteria
-A single- or double-stranded nucleic acid (DNA or RNA) surrounded by a protein shell called a capsid
-Some viruses also have an outer envelope composed of lipids and proteins.
-Inject dna into cell-
Protozoa
-flagella for locomotion.
-Unicellular
-Eukaryotic
-Reproduction is by binary fission (longitudinal division)
Yeast
-Most reproduce asexually/budding
-Eukaryotic
-Single celled
Arthropod
-Exoskeleton
-Their body is covered by a cuticle or pellicle.
-Freshwater forms have a contractile vacuole.
-Reproduction is by internal fertilization
-Multicellular
Helminths
-Invertebrates characterized by elongated, flat or round bodies
-Reproduce with eggs
Endosome pH
6
Lysosome pH
4
Recall the Frenemies classwork. Describe one scientist that you studied. Include the following information: when and where they worked, their primary contributions to microbiology, why that contribution is important, and any controversies that they were involved in.
-Dr Harold Amos
-Discovered penicillin
-First broad spectrum antibiotic
-Controversy with deserved nobel prize since others worked on penicillin mold
-Harvard, 1940s (?)
Recall the Biology & Order: Special Microbes Unit project. Describe your microbe. Include the following information: what kind of agent of infection it was (bacteria, virus, protozoan, fungi, helminth) and how you know (distinguishing features of that agent), what disease it caused and the symptoms/severity, as well as the treatment/cure/vaccines that are available.
-Yersinia pestis
-Gram negative bacillus bacteria
-Causes the black death, 50-60% mortality if untreated
-Causes fever, chills, extreme weakness, abdominal pain, shock, buboes (bubonic)
-Killed ⅓ of europe
-Treated with antibiotics
What did we observe in the chemiluminescence lab?
Temperature can affect the rate of chemical reactions
Glycolysis begins with _____
1 glucose
Glycolysis produces _____
2 ATP net, 2 pyruvate, 2 NADH
Kreb cycle begins with _____
2 Acetyl CoA
Kreb cycle produces _____
3 NADH, 2 FADH2, 2CO2
Electron transport chain begins with _____
3 NADH 2FADH2
Electron transport chain produces _____
32 ATP+H20
