EXAM I TISSUE SLIDES; ENJOY! :) Flashcards by Erin O

Identify tissue type & location

What type of cells are in this tissue (white cells)?

Epithelium
Simple Columnar
Ileum
Goblet cells

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ID: Tissue Type & Location

Epithelium
Simple cuboidal
Kidney

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ID Tissue Type & Location, There are two compartments here, what are they?

Epithelium and Connective Tissue (left portion)
Non-keratinized Stratified Squamous

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ID Tissue Type & Location. What is the bottom portion? What type of stain is used?

Epithelium
Transitional (dome-shaped cells)
Ureter
Connective tissue (bottom)
H&E Stain (Hematoxylin stains nuclei dark purple, Cytoplasm is stained pink)

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ID Tissue Type

CT
Dense Bone

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ID Tissue Type

CT
Spongy/Cancellous Bone

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ID Tissue Type

CT
Adipose

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ID Tissue Type

CT
Hyaline Cartilage

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ID Tissue Type

Muscle Tissue
Skeletal Muscle

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ID Tissue Type

Muscle Tissue
Skeletal Muscle

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ID Tissue Type

Muscle Tissue
Cardiac Muscle

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ID Tissue Type & Location

Muscle Tissue
Smooth Muscle

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ID Tissue Type & Location; What are the lines pointing to?

Nervous Tissue
Cerebral Cortex
Labeled: Neurons

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ID Tissue Type & Location

Nervous Tissue
Purkinje Cell

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ID Tissue Type & Location, what is the arrow pointing to?

Kideny
Label: lumen
Collecting duct

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ID Tissue Type & Location

Kidney
Proximal Convoluted Tubules
Simple Cuboidal

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What type of stain is used here?

pH Staining on Paramecium
Fast green at pH 2.5
This just shows what different pH demonstrates using same staining technique

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What type of stain is used here?

pH Staining on Paramecium
Fast green at pH 8.0
This just shows what different pH demonstrates using same staining technique

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What type of tissue is this?
What type of staining technique?

Adipose tissue (low mag. 100X)
Osmium Tetroxide

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What type of tissue? What staining technique is used?

Adipose tissue (high mag. 400X)
Osmium Tetroxide

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What type of tissue?
Staining technique used and what organelle is stained?

Liver
Schiff reagent
Stains nuclei pink (reacts with aldehyde groups)

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What type of tissue?
Staining technique?

Liver
PAS staining (Periodic acid-Schiff)
Arrow pointing at glycogen deposits - deep pink

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Type of tissue?
Staining technique?
What is stained here?

Liver
Best Carmine
Stains glycogen deposits - dark pink dots

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Tissue type?
Stain?

Liver
Best Carmine
Stains glycogen - dark pink (not the purple)

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* Tissue type? * Stain?

* Liver * RNA stained with pyronin methyl green * Basic dye (DNA & RNA are basophilic)

Tissue? Stain?

* Pancreas * Immunocytochemical Stain * Anti-glucagon (alpha cells more centered) * Direct labeling of Antibody conjugated with fluorescent dye

ID Tissue Type Stain?

* Pancreas * Immunocytochemical Stain * Anti-Insulin (beta cells more peripheral than glucagon)

Tissue? Stain?

* Pituitary * Immunocytochemical * Anti-ACTH

Tissue? Stain?

* Tonsil * Immunocytochemical Stain * Anti-IgG

Tissue type?

* Stratified squamous lightly keratinized * Skin

* What is labeled? * Tissue type?

* Desmosomes * Stratified squamous epithelium * Epidermis

Tissue type?

Simple Squamous

Tissue type? What are the clear cells called?

* Pseudostratified columnar epithelium * Goblet cells * Note the multiple layers of nuclei found at different levels in a single layer of cells

Tissue type? Where is this tissue located?

* Transitional Epithelium (notice the dome shaped cells) * Ureter; Urothelium

Tissue type? Location?

* Simple cuboidal * Kidney

Tissue type?

Stratified squamous non-keratinized

Tissue type? Location?

* Simple cuboidal * Kidney

Tissue type?

* Simple Columnar * Note the microvilli

Tissue type? Location?

* Transitional * Urothelium * Urinary tract

Tissue type? Location?

* Columnar (notice the brush border) * GI tract

Tissue type? Location?

Simple cuboidal; Kidney

Tissue type? Location?

* Transitional (notice the dome shaped cells) * Urothelium; Urinary tract

Tissue type?

Simple cuboidal

Tissue type?

* Simple columnar * Notice the uniform lining, making it simple (circled)

Tissue type?

* Transitional * Urothelium

Tissue type?S

* Simple columnar * Notice the goblet cells; which are usually in intestinal epithelium = columnar

Tissue type?

* Pseudostratified with microvilli

Tissue type?

* Stratified columnar

Tissue type?

Non-keratinized stratified squamous

What type of gland and what is occurring at the arrow?

* Sebaceous gland * Cells are breaking down to release their product (thick, oily material)

Type of gland? What is circled?

* Sublingual gland (contain mucous acini **ONLY**) * Duct * Mucous acini are pale in appearance = **PAS+** * Contain **Myoepithelial cells** (surround acini)

Type of gland? What are the arrows pointing to?

* Parotid gland; Serous acini **ONLY** * Apical region = zymogen granules * Basal region = RER

Tissue Type

Unilocular Adipose Tissue 400x

Tissue type? What are the arrows pointing to?

* Submandibular gland (**BOTH** serous and mucous cells, and myoepithelial cells) * Top left = ducts * Bottom left = serous acini * Botton right = mucous acini

Tissue Types?

Major Is Unilocular Adipose Tissue 100x bottom Left corner displays Fibrous Connective Tissue

Tissue Type

Elastic Connective Tissue xs, 100x

Tissue Type

Elastic Connective Tissue Is, 100x Stains pink w/ H and E

Tissue Type

Areolar CT 400X Mostly dark lines = Elastic Fiber background includes collagen fibers if look carefully

* Type of gland? * Label arrows

* Parotid gland * Left arrow = Connective tissue * Right arrow = serous acini

Tissue Type

Areolar Connective Tissue see elastic fibers background would include collagen fibers but not see well

Gland type?

Parotid gland

Type of gland? Label arrows

* Sublingual gland * Left arrow = **striated duct** * right arrow = **mucous acini**

Tissue Type

White Fibrous Connective Tissue 100x Better slide of Dense Regular Connective Tissue see parallel and regular distribution see very thin rows of nuclei

Gland type?

* Sublingual gland * Note that nuclei are located around the bases of the mucous acini

Gland type? Label arrows

* Sublingual gland * top left = **mucous acini with basal nuclei** * bottom left = **serous acini** * disregard right arrow

Tissue Type

Dense Regular Connective Tissue, 400x

Tissue Type

Plasma Cells peripheral distribution of lumps of DNA showing basically through a clock

Gland type? Label arrows

* Sublingual gland (400X) * Top = **secretory acini** * Middle = **myoepithelial cells** * Bottom = **intralobular duct;** *striated duct* (smaller diameter than Interlobular duct)

Type of gland?

Sublingual gland

Type of gland? Label arrows

* Submandibular gland * Left = **serous acini** * Right = **Interlobular duct;** *simple cuboidal duct* (larger within the CT and larger diameter than serous acini)

Tissue Type

Plasma cells clock-like representation of DNA all in delicate connective tissue on either side is a simple columnar epithelial tissues In the Gut

ID what used to take slide What slide is depicting and the 3 labels of cell structures (A, B, C)

Plasma Cell TEM slide A. Rough Endoplasmic Reticulum containing immunoglobulin molecules B. Cartwheel Nucleus C. Region of the Golgi Apparatus

Type of gland? Label arrows

* Submandibular * Left = **striated duct** * Right = **serous acini**

Gland type? Label arrows

* Parotid gland * Left = **Interlobular septum** (in between CT partitions) * Right = **Interlobular duct** (INTER = between CT) * The mucous acini looking cells are actually adipose cells = older patient

ID Tissue Type and 4 labeled structures

Plasma Cell A. Golgi Region B. Cartwheel Nucleus C. Rough Endoplasmic Reticulum D. Nucleus of a Fibroblast

Type of gland?

* Parotid * Notice it is surrounded by CT; Interlobular duct

TEM of a Mast cell

Gland type? What is the arrow pointing at?

* Submandibular gland * Blood vessels surrounding duct

ID slide

Stain: Toluidine Blue Dark Puple sctructures = Mast Cells Nuclei stain lightly in Mast cells A = Muscle Cell below mast cells is Fibrouc Connective tissue

Gland type?

Submandibular gland

Gland type?

Submandibular gland

Gland type?

Submandibular gland

Gland type?

* Mammary gland * No secretory units, therefore inactive gland

Gland type?

* Mammary gland * No secretory units = inactive

TEM of a Mast Cell with cytoplasmic granules of different density

ID the labeled structures

A. Mast Cell with metachromatic granules in the Cytoplasm B. Blood Vessel Stain: Toluidine Blue

Macrophages Stain with India Ink (very black ink made with carbon fibers) They digest the ink so it makes them easy to find Dark spots = undigestable India Ink = inside Macrophage

Machrophage Stain: Toluidine Blue Fiber in Background Yellow arrow : Fibroblasts Red Arrows : cells w/ clear spots are fibrocytic vesicles and shows phagocytosis A and B. Macrophages C. Fibroblast D. Collagen

ID slide and labeled structures

TEM of Marcrophage A. Nucleus B. Mitochondria C. Vesicle D. Secondary lysosome E. Primary lysosome F. Filopodia Lots of vesicles and process that stain the cell.. Acidic, Lots of Mitochondria Activity

Fibrocytes see some semblance of the fiber cells but fibrocytes pick up most of the stain

Fibrocytes A. Collagen fibers in cross-section B. Fibroblast in active site c. collagen fibers in longitudinal section

Adipose 100x Unilocular Fibrous connective tissue in the corner

White Fat Unilocular Adipose Tissue When can be seen...nuclei pushed to side cells empty due to process used to preserve tissue (alcohol dissolve)

Brown Fat Multilocular Adipose Tissue has lots of mitochondria

Tendon and Muscle 400x Tendon not stretched still displays the dense compactness

Reticular Silver Stain Found in Lymph node in the spleen Dense irregular CT with predominance of reticular fibers

Elastic Connective Tissue Arrows to Squiggly = Lamellae Dense type of irregular CT with an adundance of elastic fibers which form discontinuous lamellae

ID Tissue and labeled structures

Dense Irregular Adult Connective Tissue No parallel organization of Collagen bundles but densely packed thicker bundles of collagen than in Loose CT found in fascia, submucosa of GI tract, and in dermis of integument A. Collagen Fibers B. Blood Vessels C. Connective Tissue cell nuclei D. collagen Fibers

White Fibrous Connective Tissue (Dense Regular CT) 100x bundles of collagen fibers relatively unstained nuclei squeezed between and see parallel organization

Dense Regular Adult CT high ratio of fibrous components to fibroblasts thicker bundles of collagen than in loose CT highly ordered bundles of collagen separated by single rows of fibroblasts found in ligaments and tendons Nuclei compressed b/w bundles (very elongated and thin) everything runs parallel

Areolar (Loose Adult) Connective Tissue 100x Collagen not really noticable here high ratio fibroblast to fibrous components Type I collagen and elastic fibers usually found beneath epithelial tissues of most organs contains fibroblasts, mast cells, macrophages, and capillaries\* \*\*\*\*no vascular tissue in epithelial but YES in CT\*\*\*\*\*

Adult Loose (Areolar) Connective Tissue Collagen not really noticable here high ratio fibroblast to fibrous components Type I collagen and elastic fibers usually found beneath epithelial tissues of most organs contains fibroblasts, mast cells, macrophages, and capillaries\* \*\*\*\*no vascular tissue in epithelial but YES in CT\*\*\*\*\*

Embryonic Connective Tissue can see fibers but thin and spider-web like found in umbilical cord and in pulp of developing teeth "Wharton's" Jelly in umbilical cord composed of some collagen and elastic fibers but mostly an abundance of extracellular matrix (usually in the form of gylcoprotein and proteoglycans)

Umbilical Cord; x.s. A. Wharton's Jelly = Embryonic CT and the nondescript area B. Placental Artery C. Allantois D. Placental Artery E. Placental Vein

Unilocular Adipose Tissue

ID tissue and labels

Adult Connective Tissue: Elastic Connective Tissue A. Elastic Fibers B. Smooth Muscle Cells

Reticular Tissue Adult -type connective tissue characteristic of lymphatic tissue silver stain

ID tissue and labels

Dense Regular Connective Tissue Adult Connective Tissue found in ligaments and tendons A. Skeletal Muscle B. Regularly arranged collagen bundles C. Fibrocyte compressed by the aligned collagen bodies

ID Tissue and labels

Dense Irregular Adult Copnnective Tissue found in dermis of the skin, submucosa of GT tract, and other sites Fibroblasts are spare separated by collagen bundles, and recognized by their oval nucelus A. Blood capillary B. Oval nucleus of a fibroblast C. Collagen bundles thick, wavy, and irregularly arranged

ID Tissue and Labels

Loose (Areolar) Adult Connective Tissue abundant elastic fibers and collagen bundles embedded in the gorund substance A. Oval nucleus of a Fibroblast B. Elastic Fibers are thin, straight, and branching C. Collagen bundles are thick and wavy

ID Tissue and labels

Embryonic Connective Tissue contains abundant extracellular matric rich in proteoglycand collagen and reticular fibers are present but nt abundant A. Nucleus of a mesenchymal cell embedded in the extracellular matrix B. Amnion