EXAM III Proteins, COH, Lipids Flashcards Preview

MLT 130 Clinical Chemistry I > EXAM III Proteins, COH, Lipids > Flashcards

Flashcards in EXAM III Proteins, COH, Lipids Deck (73)
Loading flashcards...
1

Normal glucose concentration of serum?

80-100 mg/dL

2

Requirements of an oral GTT

Fasting at least 10 hours
Done in morning
no eating, drinking, smoking or exercise
normal to high COH intake 3 days prior

3

Order of mobility of serum proteins during an electrophoresis

albumin(fastest)-a1-a2-beta-gamma

4

Why can the biuret method NOT be used for determining CSF or urine protein?

it lacks sensitivity to detect low levels of protein which are in those specimens

5

What does the biuret reagents specifically react with?

tripeptide
2 or more peptide chains

6

What is the reference method for total protein determination?

Kjeldahl

7

Which method for measuring albumin is most common in automated instruments?

Dye-binding

8

What SPE abnormality is seen in multiple myeloma?
In nephrotic syndrome?

Spike in gamma region
^ a2 and beta region, decrease albumin

9

Glucose in CSF is approximately ____ of the amount of glucose in the blood.

2/3
60-70%

10

In bacterial meningitis, what would be the expected results for CSF protein?
CSF glucose?

protein increased
glucose decreased

11

What anticoagulant is used to prevent glycolysis?

sodium fluoride

12

Increases blood glucose levels
inhibits glucose uptake by the tissues
increases glucose output by the liver

ACTH
growth hormone

13

Increases blood glucose levels
Increases glycogenolysis and gluconeogenesis

Glucagon

14

Increases blood glucose levels
Increasing gluconeogenesis and inhibiting glucose metabolism in peripheral tissue

Glucocorticoids

15

Decreases blood glucose levels
by decreasing glycogenolysis-only hormone that decreases

Insulin

16

Increases blood glucose levels
fight or flight
stimulates glycogenolysis

Epinephrine

17

What is the action of the enzyme glucose-6-phosphate dehydrogenase?

removes hydrogen from glucose-6-phosphate and gives it to NADP

18

What technique is used to ID patients with familial hyperlipoproteinemia?

electrphoresis

19

normal value for albumin

3.5-5.0 g/dL

20

normal value for globulin

2.5-3.5 g/dL

21

normal value for TP

6.0-8.5 g/dL

22

What phenom causes the gamma globulins to move toward the cathode in SPE, even though they are negatively charged?

EEO
electroendosmosis

23

What specific form of hgb is used to monitor patients with Type I diabetes mellitus?

A1c

24

How is globulin in serum routinely determined?

TP - albumin

25

Which fraction of lipprotein contains highest percentage of protein?

HDL

26

What is the order of migration of lipoproteins?

chylomicrons - prebeta - beta - alpha

27

What is the desirable TC level in serum

less than 200 mg/dL

28

Most likely explanation for a patient with lab results of elevated TC and TGD but normal lipoprotein pattern?

patient isn't fasting
non fasting specimen

29

Which lipoprotein fraction is responsible for post-prandial lipemia?

chylomicrons

30

If plasma is used instead of serum for electrophoresis, what change will be observed?

Spike in beta gamma region
due to fibrinogen