Exam IV Non-nitrogen Protein, Kidney & Liver functions, Enzymes, Cardiac markers Flashcards Preview

MLT 130 Clinical Chemistry I > Exam IV Non-nitrogen Protein, Kidney & Liver functions, Enzymes, Cardiac markers > Flashcards

Flashcards in Exam IV Non-nitrogen Protein, Kidney & Liver functions, Enzymes, Cardiac markers Deck (98)
Loading flashcards...
1

What is the substrate for the enzyme aspartate amino transferase (AST)?

aspartate
alpha ketoglutamic acid

2

Creatinine is formed from the breakdown of?

creatine

3

PSP is removed from the blood by the?

kidney

4

What is the principle of the Berthelot reaction and what substances can cause falsely elevated values in the reaction?

hydrolysis of urea into ammonium carbonate by the enzyme urease; cause blue colored compound

high levels of ammonia or bilirubin

5

Increased ALT values are associated with disease of?

liver

6

What is the normal value for serum AST at 37?

5-35 uL

7

Which anticoagulant should NOT be used for the urease testing?

sodium fluoride

8

What is the normal percentage for the CK-MB isoenzyme?

less than 6%

9

What reagent is used in the measurement of uric acid?

phosphotungstic acid

10

Creatinine clearance is used to assess the rate of?

glomerular filtration

11

Crcl equation

(Ucr * Vu)/(Pcr * t)

12

What additional info must be know to calculate Crcl if the patient is an infant, young child or adolescent?

body surface area

13

What is the most common endogenous substance used to assess GFR?
Exogenous substrate?

creatinine clearance
inulin

14

Serum creatine kinase is most often used in diseases of?

muscle tissue

15

Serum lipase levels are elevated in disease of?

pancreas

16

Diacetyl monoxime reacts with ? to produce a yellow product?

urea

17

What is uremia?

abnormal substances of urea in the blood

18

What is the normal value for CK in males at 37?

15-160 U/L

19

Three ways that can be used in the lab to measure the rate of an enzymatic reaction.

Multiple point assay
Continous Monitoring
Kinetic point assay

20

Define the International Unit (IU)

quantity of enzyme that will catalyze one micromole (umol) of substrate per minute

21

What is the peak absorbance of reduced NAD (NADH)?

340nm

22

The Jaffe reaction is used to measure?

creatinine

23

Which non-protein nitrogen component comprises the largest fraction of NPN in the blood?

BUN/ urea

24

Which enzyme is useful in the detection of insecticide poisoning?

pseudocholinesterase

25

Low levels ow wchich enzyme can cause a patient to be susceptible to prolonges apnea when exposed to certain types of anesthesia?

pseudocholinesterase

26

Increased levels of LD-a and LD-5 are associated with?

liver
acute hepatic disease

27

Gout is caused by the deposition of ? in joints and other tissues.

uric acid

28

What is the cilnical significance of a mildly elevated ALP in a teenager?

No significance: still growing

29

What is the normal ration of BUN to creatinine?

10:1 to 15:1

30

What enzyme is normally elevated during pregnancy?

alkaline phosphotase