Exp. 5 & 6_Post Lab Flashcards

(39 cards)

1
Q

stores the genetic information of an organism

A

DNA

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2
Q

transmits that information from one generation to another.

A

DNA

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3
Q

translates the genetic information contained in DNA into proteins needed for all cellular function

A

RNA

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4
Q

Responsible for protein synthesis

A

Nucleic Acid

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5
Q

A nucleic acid is an

A

unbranched polymer

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6
Q

monomer units of nucleic acids are

A

nucleotides

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7
Q

A___________ is an unbranched polymer in which the monomer units are nucleotides.

A

nucleic acid

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8
Q

T or F| ALL nucleic acid molecules are unbranched polymers.

A

Trueee!!!

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9
Q

A nucleotide is composed of:

A

Nitrogen-containing bases (amines)
Sugar
Phosphate

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10
Q

T or F| ATP is an example of a nucleotide

A

TRUEEE

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11
Q

What is ATP composed of?

A

Adenine
3 phosphate
Ribose

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12
Q

Nitrogen containing bases

A

Purines
Pyramidines

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13
Q

2 ringed nitrogen group

A

Purines

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14
Q

1 ringed nitrogen group

A

Pyrimidines

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15
Q

Purines of DNA and RNA

A

Adenine
Guanine

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16
Q

Pyrimidines of DNA

A

Cytosine
Thymine

17
Q

Pyrimidines of RNA

A

Cytosine
Uracil

18
Q

Sugar of RNA

19
Q

Sugar of DNA

20
Q
  • crush cell wall
21
Q

Homogenizing solution

A

Salt and Dishwashing liquid

22
Q
  • removes the lipids and push DNA to the salt solution
A

dishwashing liquid

23
Q
  • increases separation of DNA from hydrophobic layer; aggregates DNA
A

sodium chloride

24
Q

– promotes aggregation of DNA and wash the DNA from the salt

25
Test for the deoxyribose in DNA
Dische Diphenylamine Test
26
Dische Test: Reagents
diphenylamine Sulfuric acid
27
Dische test positive results
light blue to blue depending on the amount of DNA
28
Dische Test: GLUCOSE
Grayish Brown
29
Dische Test: RIBOSE
Greenish brown
30
Dische Test: DEOXYRIBOSE
Bright Blue
31
Dische Test: EXTRACTED DNA
Brownish Blue
32
Hydrolysis of DNA molecule
Acid Hydrolysis
33
breakdown of glycosidic bonds only if pH > 3
Depurination
34
Depurination breakdown of glycosidic bonds only if pH is
pH > 3 More than 3
35
Products of Depurination
purine base deoxyribose with phosphate group
36
breakdown of glycosidic bond with phosphodiester bond if pH < 2
Total breakdown
37
Total breakdown breakdown of glycosidic bond with phosphodiester bond if pH is
pH < 2 Less than 2
38
Extremely low pH_____ DNA completely
hydrolyses
39
Products of total breakdown
phosphate group purine or pyrimidine base deoxyribose