EXPERIMENT #1 AND 2 Flashcards by MARY ANGELICA JACILDONE

An essential tool in studying of microorganisms, cells and tissues

Microscope

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An instrument used to contain reagents as well as parasites for analytical identification

Glasswares

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QUALITY CONTROL:
True or False for Binocular Microscope
Inspect the microscope before and after use

True

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QUALITY CONTROL:
In using the binocular microscope, we should clean the objectives ______________ of using the microscope with _____________ only

After each day
Cleaning tissue

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QUALITY CONTROL:
In using the binocular microscope, we should never use ________, _________, __________, ________________, ______________ or ___________ since it can cause damage or scratch to the lens surfaces

Alcohol, ordinary tissues, cleaning paper, toilet paper, cotton wool or hand towels

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QUALITY CONTROL:
In using the binocular microscope, we should store the microscope in a _____ condition to prevent ____________________ on the glass surface

Dry condition
Fungal growth

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QUALITY CONTROL:
In using the binocular microscope, in realigning the optics, replacement of lens and maintenance and lubrication of the stage should be performed by whom?

Qualified Service Technician

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QUALITY CONTROL:
In using the Clinical centrifuge, the external part of the centrifuge should _________ clean with ________ agents in order to prevent residual contamination

Regularly clean
Neutral agents

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QUALITY CONTROL:
In using the Clinical centrifuge, we should always check the rotor and lid for scratches, dust, or spilled sample _________ and _________ use.

Before and after use

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QUALITY CONTROL:
In using the Glass slides and coverslips, the specimen side of the glass slide should be kept ___________ or _____

Horizontal or flat

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QUALITY CONTROL:
In using the Glass slides and coverslips, glass slides should always be stored in a _____________ designed for its purpose, away from ______ and _________ light

Container
Heat
Bright light

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QUALITY CONTROL:
In using the Glass slides and coverslips, the suitable place in storing the glass slide is a

Cool, dark place such as locked cabinet in a temperature controlled room

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QUALITY CONTROL:
In using the Glass slides and coverslips, in cleaning the contaminated slides we should use

Soapy water or Isopropyl alcohol

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QUALITY CONTROL:
True or False for Glass slides and coverslips, Slides should not be immersed or soaked in water as it loosens the glue on the cover glass

True

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QUALITY CONTROL:
For Centrifuge tubes, it is considered as the primary source of sample leakage protection and must be lubricated before or after installing a new motor

O-rings

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QUALITY CONTROL:
For Centrifuge tubes, in using o-rings ensure the optimal performance and to prevent cross-threading and corrosion, threaded components should be cleaned and oiled on a __________

Daily basis

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QUALITY CONTROL:
True or False for Centrifuge tubes, the tubes are compatible with the fluids used, size compatible with rotor, and the mass of the opposing tubes to be centrifuged are balanced

True

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QUALITY CONTROL:
In using Plain Test Tubes, check the ______ of the test tube for any dents ______ and ______ use

Edges
Before and After use

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QUALITY CONTROL:
In using Plain Test Tubes, soak the test tube with what solution?

Detergent solution

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QUALITY CONTROL:
In using Plain Test Tubes, wash the glassware with _________________ by using a _________ brush

Running tap water
Nylon Brush

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QUALITY CONTROL:
In using Plain Test Tubes, place it in a test tube rack with their mouth facing _____________

Downwards

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QUALITY CONTROL:
In using Beakers, always check the beaker for any breakage _______ and _______ using

Before and after use

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QUALITY CONTROL:
In using Beakers, we should store them in a ________ to protect from __________. We can also make use of _____

Cabinet
Dust
Cotton

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PARTS OF THE BINOCULAR MICROSCOPE:
This holds the eyepiece and objective lenses

Microscope Head

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PARTS OF THE BINOCULAR MICROSCOPE: It is allowed to be adjusted for the height and comfort of the user

Microscope Head

PARTS OF THE BINOCULAR MICROSCOPE: It connects the eyepiece to the objective lense and holds them in place

Binocular Tube

PARTS OF THE BINOCULAR MICROSCOPE: Connects the head to the base and holds it in place that provides a stable and secure support for the head of the microscope

Arm

PARTS OF THE BINOCULAR MICROSCOPE: It is used as a lense that the user look through and views the sample

Eyepiece

PARTS OF THE BINOCULAR MICROSCOPE: Closest to the sample and are used to magnify the image. Allows the user to see small details and structure that would be visible to the naked eye

Objective Lenses

PARTS OF THE BINOCULAR MICROSCOPE: Holds the objective lenses and allows them to be rotated

Nosepiece

PARTS OF THE BINOCULAR MICROSCOPE: Provides a light source for illuminating the sample

Illuminator

PARTS OF THE BINOCULAR MICROSCOPE: A platform on which the sample is placed

Stage

PARTS OF THE BINOCULAR MICROSCOPE: It is a small opening or hole that is used to control the amount of light that passes through the microscope to optimize the image quality and minimize glare or reflections

Aperture

PARTS OF THE BINOCULAR MICROSCOPE: It is used for focusing the light onto the sample

Condenser

PARTS OF THE BINOCULAR MICROSCOPE: Used to control the amount of light that passes through the microscope

Diagphragm

PARTS OF THE BINOCULAR MICROSCOPE: Used for adjusting the distance between objective lenses and sample

Focus mechanism

PARTS OF THE BINOCULAR MICROSCOPE: Used to hold the sample and move into position for viewing

Mechanical Stage

PARTS OF THE BINOCULAR MICROSCOPE: Small device that used to hold the sample securely in place

Stage clips

PARTS OF THE BINOCULAR MICROSCOPE: Sits on the surface and supports the rest of the microscope

Base

PARTS OF THE BINOCULAR MICROSCOPE: A switch that turns the illuminator on and off

On / Off switch

PARTS OF THE BINOCULAR MICROSCOPE: It is used to control the movement mechanism stage

Stage controller

PARTS OF THE BINOCULAR MICROSCOPE: A lever that is used to fine-tune the focus of the microscope

Fine adjustment

PARTS OF THE BINOCULAR MICROSCOPE: Allows the user to make a small, precise adjustments to the focus of the microscope, necessary for producing clear and detailed image

Fine adjustment

PARTS OF THE BINOCULAR MICROSCOPE: It is used to focus the microscope by moving the objective lenses relative to the sample

Coarse Adjustment Knob

PARTS OF THE BINOCULAR MICROSCOPE: Controls on how far the stages should go preventing the objective lenses from getting too close to the specimen slide which may damage the specimen

Rack stops

A residual mass of material remaining in the intestinal tract after the full and complete exercise of the digestive and absorptive functions and are ultimately expelled from the body through the rectum.

Feces

The semi-fluid intestinal contents at the ________________ are transformed into feces in the _________________ where the residues remain for one or more days

Duodenal valve Large Intestines

PROPER COLLECTION OF STOOL: Stool containers should be __________ (to avoid contamination) , _________, ____________ (for easy collection ) and preferably __________ (for physical examination . It should be free from ________, _______ and ________

Covered Clean Wide-mouthed Colorless Free from water, urine, and soil

PROPER COLLECTION OF STOOL: TIME 1. Submit the specimen ____________ to the laboratory and see to it that it is completely and properly labeled. 2. Stool examination falls under _______________________________in a hospital set-up and thus may not be given priority since there are other specimens to be examined.

1. Immediately 2. Clinical Microscopy

PROPER COLLECTION OF STOOL: Diagnosis of amoebiasis 1. DIARRHEIC OR WATERY STOOLS must be examined within (a) _________ since (b)________ die within period of time. This (c) ____________________ is sensitive to environmental changes 2. FORMED: ________ up to _______ but we should make sure that it is preserved and refrigerated

1.(a) 30 minutes, (b) & (c) Trophozoites 2. 1 hour to 24 hours

PROPER COLLECTION OF STOOL: Preservatives used are - Refrigerate 4-8 degree Celsius for 2-3 daysit should not put the stool in the freezer because the trophozoites will die and the structures of the eggs will be destroyed.

10%formalin solution Polyvinyl alcohol (PVA).

PROPER COLLECTION OF STOOL: Preservatives used are

10%formalin solution Polyvinyl alcohol (PVA).

PROPER COLLECTION OF STOOL: TIME Refrigerate ______________________ for ____________ it should not put the stool in the freezer because the _________ will die and the structures of the eggs will be destroyed.

4-8 degree Celsius 2-3 days trophozoites

PROPER COLLECTION OF STOOL: AMOUNT For routine stool examination, 1. ________ of formed stools (______ / _____ size) 2. ________ of watery stools will suffice 3. Used for the Recovery helminthic adult. This is best exemplified by the examination done after ________ treatment.

1. 2-5 g, walnut / thumb size 2. 5 -6 tablespoon 3. Whole stool, Taenia species

FORMATION OF STOOL: Mouth →(b) ______ → (c) ___________ → (d) __________ → (e) _____________ → (f) _______

Mouth → Stomach → S Intestine → L Intestine → Rectum → Anus`

FORMATION OF STOOL: MOUTH Food + __________ formation of ________

Saliva amylase Bolus

FORMATION OF STOOL: STOMACH It breakdown the food to small pieces and have contact with ________

digestive juices

FORMATION OF STOOL: A site where the absorption of the nutrients that we eat and drink

Small Intestine

FORMATION OF STOOL: A site where it absorbs Salt and Water making the poopie firm

Large Intestine

FORMATION OF STOOL: RECTUM Process of move from Large Intestine to Rectum is called __________ (muscle contraction)

peristalsis

FORMATION OF STOOL: Site where the poops will pass through and says bye bye see yah again

Anus

What is the importance of the proper collection of fecal samples?

For accuracy result , Proper diagnosis

What is the importance of delivering the stool specimens in the laboratory as soon as possible?

morphologic forms of the protozoa and helminths

Why stool should not be mixed with toilet water and urine? These two may be confused with human parasites

Free-living protozoa and nematodes

IMPOROPER COLLECTION: 1. Immediate collection of patients with medication ______, ________ (known to have crystal residue and pwede mag iwan ito sa stool) or _________ (5-7 days after the completion bago ulit kumuha) 2. antibiotic/antimalarial - collect _______ or _______ medication 3. specimen with no proper/label – (Last Name, First Name, M.I., Identification no., time of collection, date of collection, age)

1. barium, bismuth, mineral oil 2. Before or after

EXPERIMENT #1 AND 2 Flashcards

(65 cards)