Experiment 10

Question 1

Method used for Total Creatine Kinase Determination?

Answer 1

Kinetic Method by Oliver-Rosalki, Modified by Szasz

Question 2

It plays an important role in the energy-storing mechanism of tissue by catalyzing the reverse reaction

Answer 2

Creatine Kinase

Question 3

CK is distributed in ________

Answer 3

Various organ

Question 4

What are the highest activities in CK (increasing order)

Answer 4

Brain-Heart-Skeletal Muscle

Question 5

The determination of CK is an aid in diagnosing:

Answer 5

muscular dystrophy
other diseases of the Skeletal Muscles
Myocardial infarction
hypothyroidism
renal diseases
dysfunction

Question 6

A modified method was described by?

Answer 6

Nielson

Question 7

What are the compound does Nielson added to assure maximum CK activity?

Answer 7

sulfhydryl compound and AMP

Question 8

What are the activity does Nielson has?

Answer 8

CK activity and ihibit andenylate kinase activity

Question 9

Optimized conditions for measuring CK were published by _____ in _____ as well as by the ________ committee on enzyme.

Answer 9

Szasz, 1976, Scandinavian

Question 10

pH of Forward in Total CK

Answer 10

6.8 pH

Question 11

pH of Reverse in Total CK

Answer 11

9.0 pH

Question 12

This is a direct or forward reaction which involves the conversion of creatine and ATP to Creatine phosphate and ADP.

Answer 12

Tanzer-Gilbarg Method

Question 13

Most Cathodal in Electrophoresis

Answer 13

Mitochondrial CK

Question 14

Cathodal in Electrophoresis

Answer 14

CK-MM

Question 15

It migrates to a position midway between CK-MM & CK-MB

Answer 15

Macro CK

Question 16

Anodal in electrophoresis

Answer 16

CK-BB

Question 17

Degree of damage to the left ventricle : SMALL MI

Answer 17

<10% damage

Question 18

Degree of damage to the left ventricle : MEDIUM MI

Answer 18

10-30% damage

Question 19

Degree of damage to the left ventricle : LARGE MI

Answer 19

> 30% damage

Question 20

Protein Mass Value : SMALL MI

Answer 20

10-60 ug/L

Question 21

Protein Mass Value : MEDIUM MI

Answer 21

60-225 ug/L

Question 22

Protein Mass Value : LARGE MI

Answer 22

> 225 ug/L

Question 23

% of CK-MM

Answer 23

96%

Question 24

CK-MM:_________ ; CK-MB:________

Answer 24

lipoprotein, Ig

Question 25

% of CK-MB

Answer 25

0-6%

Question 26

% CK-BB

Answer 26

small amount

Question 27

In Column Chromatography, it exchange in resin.

Answer 27

diethyl amino ethyl

Question 28

The creatine formed in the reverse reaction is estimated fluorometrically after addition of ____?

Answer 28

ninhydrin

Question 29

The rate of formation and utilization of ATP in the CK assay can be followed continually by bioluminiscence reaction with _____\_____.

Answer 29

Luciferin,luciferase

Question 30

Excesses in the reaction system that may inhibit the activity of CK.

Answer 30

Magnesium, Chloride, Calcium, and Sulfates

Question 31

Other heavy metals that also inhibits the activity of CK

Answer 31

zinc,copper,manganese

Question 32

Excess _____ & ______ agents

Answer 32

iodoacetate , sulfhydryl

Question 33

What are the excesses that inhibit the activity of the enzyme?

Answer 33

ADP(Adenosine Di-Phosphate) Citrate fluoride L-thyroxine Uric acid

Question 34

Serum stability:

Answer 34

4hrs-Room temp 8-12hrs-4 Degree Celcius 2-3 days- Frozen

Question 35

hemolyzed specimens should not be used, what are the side reactions tha may occur?

Answer 35

-Adenylate kinase -Adenosine triphosphate -Glucose-6-phosphate dehydrogenase

Question 36

Where adenylate kinase occur?

Answer 36

Erythrocytes Platelets

Question 37

Adenylate Kinase may also occur in what tissues?

Answer 37

Skeletal Muscle Brain Liver Heart Kidney Small Intestine

Question 38

Linearity of Total CK

Answer 38

1200IU/L

Question 39

serves as the cofactor for the maximum catalytic activity of enzyme

Answer 39

Magnesium

Question 40

inhibits adenylate kinase activity

Answer 40

Adenosine-5’-Monophosphate

Question 41

prevents oxidation of sulfhydryl group of creatine kinase

Answer 41

N-acetylcysteine

Question 42

Chelates calcium which is an inhibitor or the enzyme’s activity

Answer 42

EDTA