Laboratory Exp. 8 AMYLASE

Question 1

What class does amylase belong?

Answer 1

Hydrolases

Question 2

Starch is composed of?

Answer 2

Amylose & Amylopectin

Question 3

It is long, unbranched, polypeptide chain with alpa-1,4-glycosidic bonds.

Answer 3

Amylose

Question 4

What bond is found in amylose?

Answer 4

Alpha-1,4-glycosidic bonds

Question 5

a branch of polypeptide chain with alpha-1,6-glycosidic bonds

Answer 5

Amylopectin

Question 6

What bond is found in Amylopectin?

Answer 6

alpha-1,6-glycosidic bonds

Question 7

It is the smallest enzyme that is readily filtered by glomerulus(renal)

Answer 7

Amylase

Question 8

Amylase can appear in?

Answer 8

Urine

Question 9

It is important enzyme in the physiologic digestion or starches

Answer 9

Amylase

Question 10

What are the major source/tissue source of Amylase?

Answer 10

Pancreas and Salivary glands

Question 11

What are the isoenzyme in amylase?

Answer 11

P-type isoamylase
S-type isoamylase

Question 12

Amylase catalyze the breakdown of _____ and _____

Answer 12

Starch & Glycogen

Question 13

Difference between glycogen and amylopectin?

Answer 13

Glycogen is more HIGHLY BRANCHED

Question 14

What is the only bond attacked by alpha-AMY to produce degradation products consisting of glucose, maltose, and intermediate chains, called dextrin?

Answer 14

alpha-1,4-glycosidic bonds

Question 15

What are the degradation products?

Answer 15

Glucose, Maltose, and Dextin

Question 16

Tissue source with lesser concentration of serum AMY

Answer 16

Skeletal muscle, small intestine, fallopian tubes

Question 17

Molecular weight of Amylase

Answer 17

50,000 to 55,000

Question 18

Stability of Specimen in Amylase?

Answer 18

1 week at Room Temperature

Question 19

What is the temperature in Several months:

Answer 19

2-8 Deg C

Question 20

Does the anticoagulant Citrate, oxalate, and EDTA can used in Amylase Specimen collection? (T/F)

Answer 20

False

Question 21

Status of serum AMY levels in axite pancreatitis: Time

Begin to rise:______
Peak:_____
Return to normal:______

Answer 21

:5-8 hrs
:24 hrs
:3-5 days

Question 22

Other disorders causing elevated serum AMY level

Answer 22

•Salivary gland lesions(mumps and parotitis)
•Intra-abdominal diseases(intestinal obstruction, cholecystitis, appendicitis)
•Renal insufficiency
•Diabetic ketoacidosis

Question 23

Cause of false increase:

Answer 23

-Macroamylasemia
-Lipemia
-Bilirubin Concentration >20mg/dL
-Insulin
-Morphine and Opiates
-Contamination with saliva and sweat

Question 24

It measures the disappearance of starch substrate

Answer 24

AMYLOCLASTIC

Question 25

Measures the appearance of the product

Answer 25

Saccharogenic

Question 26

Measures the increasing color from production of product coupled with a chromogenic dye

Answer 26

Chromogenic

Question 27

Coupling of several enzyme systems to monitor amylase activity

Answer 27

Continuous Monitoring

Question 28

Methods to separate isoenzyme:

Answer 28

Physical Characteristics Chromatography Electrophoresis Electric forms Immune inhibition Immune precipitation selective inhibition

Question 29

Where is P isoamylase derived from?

Answer 29

Pancreatic tissue

Question 30

Where is S isoamylase derived from?

Answer 30

Salivary glands, Fallopian tube, & Lung

Question 31

Method of Amylase

Answer 31

Kinetic Method by wallenfels et al

Question 32

He introduced iodometric method in 1908?

Answer 32

Wohlegemuth

Question 33

He introduced a procedure that standardized the amount of starch and iodine? What year?

Answer 33

Somogyi,1938

Question 34

Somogyi’s work became the basis for the widely used _______ & __________ methods in 1956 & 1960

Answer 34

Amyloclastic and Saccharogenic

Question 35

What did Wallenfels et al introduced?

Answer 35

p-nitrophenylglycosides

Question 36

a substrate that terminal glucose blocked to reduce spontaneous degradation

Answer 36

p-nitrophenyl-D-maltohepataoside(PNPG7)

Question 37

Reduce spontaneous degradation of the substrate by:

Answer 37

glucosidase & glucoamylase

Question 38

Convert Somogyi units to IU

Answer 38

x 1.85

Question 39

Linearity of Amylase Determination

Answer 39

1500IU/L

Question 40

Factor in Amylase

Answer 40

4824

Question 41

Expected Value

Answer 41

up to 96 IU/L