extra

Question 1

what is the same bw RCT and cholesterol efflux asssays

Answer 1

the same equilibiration steps and labeling steps

Question 2

what happens to macrophages in a RCT assay

Answer 2

injected into the peritoneum

Question 3

what is studied over the 48 hrs in RCT assay

Answer 3

feces and blood

Question 4

what is studied after 48 hrs in a RCT assay

Answer 4

blood, bile, tissues

Question 5

how much cholesteorl is in the brain

Answer 5

25% of the total mass of entire body cholesterol content

Question 6

how are lipids transported in the brain

Answer 6

lipoproteins

Question 7

what lipoprotein is not foudn in th ebrain

Answer 7

lpB

Question 8

what lp is in the brain

Answer 8

ApoA-I- 0.5% of bloodstream levels due to tranfer through BBB

ApoE synt and secreted IN CNS by astrocytes and microglia

Question 9

what is brain APOE used for

Answer 9

to produce APOE-HDL

Question 10

migration type of APOE

Answer 10

gamma migrating

Question 11

where is apoE found

Answer 11

almost exclusvely in the brain

Question 12

what does apoE contain

Answer 12

unesterified C and PL

Question 13

what type of PL is in apoE

Answer 13

phosphatidyl ethanolamine and not th enormal plasma phosphatidylcholine

Question 14

what form of epoE is secreted

Answer 14

8nm intact from cells and ABCA1 transfers lipid to make it bigger

Question 15

abca1 ko mice

Answer 15

have 20% norma apoE levles and microgliall cells accumulate lipid

means that apoe 8nm is rapidly removed

LDLR reduced in microglial cells

Question 16

LDLR ko mice

Answer 16

80% apoE reduction

Question 17

benifica and detrimental apoE isoform

Answer 17

E2- benificial
E4- detrimental