F, Lab (katokatz)

Question 1

Type of feces used

Answer 1

formed/soft stool

Question 2

Advantages

Answer 2

– little technical time
– simple
– no centrifuge
– large amount of specimen

Question 3

Disadvantages

Answer 3

– not satisfactory for protozoa or helminth larvae
– does not work well with watery stools or feces with
large amount of gas

Question 4

Wetting solutions

Answer 4

Malachite green and glycerin

Question 5

staining the background to make a contrasting
environment for easy visualization of the number of
eggs present in the stool

Answer 5

Malachite green

Question 6

– clearing solution
– dissolves fecal debris

Answer 6

Glycerin

Question 7

Clearing process takes how long of RT

Answer 7

1hr

Question 8

Clearing process must be incubated in

Answer 8

140c

Question 9

Clearing process heat of a

Answer 9

50-watt light bulb for 20-30 minutes

Question 10

Precautions for clearing process

Answer 10

– smears should be turned up to dry
– determine local condition for effective clearing time
– thin-shelled become invisible when smears are cleared too
long (hookworms)

Question 11

determine intensity of helminth’s infection, cure rate (CR),
Egg-reduction rate (ERR) after treatment

Answer 11

QUANTITATIVE TECHNIQUES

Question 12

Methods for quantitative techniques

Answer 12

1. Kato Katz Modified Thick Smear
2. Stoll Egg Count

Question 13

Stool egg count

Answer 13

Number of eggs x 100 = total eggs/cc of formed feces

Question 15

Conversion of stool egg count

Answer 15

Normally formed x 1
Mushy x 2
Diarrheic x 4

Question 16

easier to concentrate and
find

Answer 16

active motile larvae

Question 17

Methods for Recovery and Hatching of Helminth Larvae

Answer 17

1. Filter Paper Method (Harada - Mori’s Method)
2. Baermann Technique

Question 18

simplest procedure to hatch and isolate larve of
helminthes for identification

Answer 18

Harada - Mori’s Method

Question 19

Harada - Mori’s Method is effective for

Answer 19

hookworm, strongyloides, and
trichostrongylus

Question 20

Based on the active migration or movement of larvae

Answer 20

Baermann technique

Question 21

simplest method to
recover strongyloides
larvae from light infection

Answer 21

Baermann Technique

Question 22

To determine if parasite is present

Answer 22

Thick blood smear

Question 23

To confirm the plasmodium spp present

Answer 23

Thin blood smear

Question 24

study of morphology of individual parasites (Malaria)

Answer 24

Thin Blood Smears

Question 25

Thin Blood Smears Disadvantage

Answer 25

fail to reveal many light infections

Question 26

Thin blood smears only work for heavy infections t or f

Answer 26

True

Question 27

reveal quickly sparse or scanty infections (new/chronic)

Answer 27

Thick Blood Smears

Question 28

Thick blood smear disadvantages

Answer 28

species differentiation is more difficult

Question 29

Thick Blood Smears Indications:

Answer 29

survey work • large number of slides for malarial exam are received

Question 30

get blood anytime because some/all stages can be found

Answer 30

Plasmodium vivax

Question 31

– in between chills – ring forms

Answer 31

Plamodium falciparum

Question 32

T or f Ring forms are difficult to differentiate between species

Answer 32

True