Fastidious GNB

Question 1

Hacek

Answer 1

Aggregatibacter aphrophilus

Question 2

hAcek

Answer 2

Aggregatibacter actinomycetemcomitans

Question 3

haCek

Answer 3

Cardiobacterium hominis

Question 4

hacEk

Answer 4

Eikenella corrodens

Question 5

haceK

Answer 5

Kingella species

Question 6

HACEK growth requirements

Answer 6

CHOC & BAP
CO2
No growth MAC or EMB

Question 7

H - Aggregatibacter aphrophilus disease

Answer 7

endocarditis

Question 8

H - A. aphrophilus factor requirements

Answer 8

XV independent

Question 9

A- A. actinomycetemcomitans disease

Answer 9

1. Endocarditis
2. Periodontitis
3. Actinomyces israelii co-infection

Question 10

A. actinomycetemcomitans colony morphology

Answer 10

star shaped

Question 11

C - Cardiobacterium hominis disease

Answer 11

Endocarditis

Question 12

C. hominis Gram stain

Answer 12

rosettes

Question 13

E - Eikenella corrodens infections

Answer 13

bites/trauma from fist fights

Question 14

K - Kingella species infections

Answer 14

bone & joint infections in young children

Question 15

Bordetella pertussis stages

Answer 15

1. Catarrhal
2. Paroxysmal
3. Convalescent

Question 16

B. pertussis selective media

Answer 16

Borget-Gengou Agar
Charcoal Blood Agar (Regan Lowe)

Question 17

Filamentous hemagglutinin (FHA) and Pertactin

Answer 17

facilitate attachment

Question 18

Pertussis toxin allows for (3)

Answer 18

adhesion
invasion
interference with immune effector cells

Question 19

adenylate cyclase toxin

Answer 19

interference with immune cells

Question 20

tracheal cytotoxin

Answer 20

ciliostasis, cytotoxicity

Question 21

B. pertussis toxins

Answer 21

1. Filamentous hemagglutinin (FHA)
2. Pertactin
3. Pertussis toxin
4. Adenylate cyclase toxin
5. Tracheal cytotoxin

Question 22

Legionella infections

Answer 22

Pontiac Fever
Legionnaire’s disease
Asymptomatic

Question 23

Legionella habitat

Answer 23

soil, water

Question 24

Legionella Gram stain

Answer 24

pleomorphic GNB

Question 25

Legionella diagnosis is done by

Answer 25

direct detection in urine

Question 26

Legionella culture

Answer 26

BCYE aerobic humidity

Question 27

Pasturella infection

Answer 27

cellulitis with lymph involvement

Question 28

Pasteurella culture

Answer 28

BAP CHOC

Question 29

Pasturella Gram stain

Answer 29

small GNB

Question 30

Pasteurella biochems

Answer 30

Oxidase pos Indole pos Catalase pos Glucose fermenter

Question 31

Brucella is transmitted by

Answer 31

contaminated food inhalation direct contact with infected animals

Question 32

Brucella Gram stain

Answer 32

GN coccobacilli

Question 33

Brucella infections are known as

Answer 33

undulant fever Bang's disease Gibraltar fever Mediterranean fever Malta fever

Question 34

Brucella cultures

Answer 34

Brucella agar BAP CHOC

Question 35

Brucella biochem

Answer 35

oxidase pos catalase pos urease pos

Question 36

You can't use automation with

Answer 36

Brucella and Francisella

Question 37

Francisella infection

Answer 37

tularemia

Question 38

Francisella requires supplementation with

Answer 38

cysteine

Question 39

Can pass through unbroken skin

Answer 39

Francisella Brucella

Question 40

Francisella biochem

Answer 40

ferments glucose X & V neg urea neg beta lactamase pos