Strep and Enterococcus

Question 1

strep and entero gram stain and biochems:
aerotolerance
catalase
oxidase

Answer 1

gp pairs/chains
aerotolerant
cat neg
oxidase neg

Question 2

strep routine media

Answer 2

BAP, CHOC, CNA, PEA

Question 3

strep/entero habitat

Answer 3

respiratory tract, gi tract

Question 4

alpha hemo strep sp (2)

Answer 4

pneumoniae, viridans

Question 5

gamma hemo entero

Answer 5

faecalis, faecium

Question 6

strep pneumo hemolysis and biochems:
optochin
bile solubility

Answer 6

alpha hemo
optochin sensitive
bile soluble

Question 7

strep agalactiae group and biochems:
Bacitracin
optochin
hippurate hydrolysis
CAMP

Answer 7

group b
bacitracin R
optochin R
hippurate hydrolysis pos
CAMP pos

Question 8

GAS diseases

Answer 8

impetigo
rheumatic fever
scarlet fever
necrotizing fasciitis
glomerulonephritis

Question 9

M protein

Answer 9

adherence, antiphagocytic, coag activation

Question 10

Streptolysin S

Answer 10

GAS hemolysis aero/anaero

Question 11

streptolysin O

Answer 11

GAS hemolysis anaerobic

Question 12

GAS toxins (5)

Answer 12

SpeA/B/C super ag
streptokinase
DNAse
hyaluronidase
plasminogen-plasmin binding

Question 13

Streptokinase

Answer 13

lyse fibrin clots by acting on plasminogen

Question 14

plaminogen-plasmin binding proteins

Answer 14

convert plasminogen to plasmin and break up clots

Question 15

post strep sequelae

Answer 15

glomerulonephritis, rheumatic fever

Question 16

glomerulonephritis

Answer 16

organism-Ab complex deposit in glomerulus binds C’ causing inflammatory response

Question 17

rheumatic fever

Answer 17

Abs x-react w/ heart tissue, autoimmune

Question 18

Strep pyogenes group and biochems:
hemolysis
PYR
bacitracin
SXT
CAMP
hippurate hydrolysis

Answer 18

GAS
beta hemo
PYR pos
A disk sus
SXT R
CAMP/hipp neg

Question 19

PYR

Answer 19

L-pyrrolidonyl beta napthylamide hydrolysis

Question 20

strep pneumo biochems:
heme
catalase
optochin
virulence

Answer 20

alpha hemo
catalase neg
optochin sus
capsule

Question 21

Enterococcus biochems:
heme
catalase
bile esculin
PYR
NaCl

Answer 21

alpha hemo
catalase neg
bile esculin pos
PYR pos
6.5 NaCl pos

Question 22

Group D strep biochems
Heme
catalase
bile esculin
PYR
NaCl

Answer 22

alpha/gamma hemo
catalase neg
bile esculin pos
PYR/6.5 NaCl neg

Question 23

Bacitracin/A disk

Answer 23

susceptible is presumptive pyogenes/group A

Question 24

Strep ID flow Beta hemo

Answer 24

1. PYR or bacitracin
2. CAMP or hippurate hydrolysis
3. bile esculin
4. PYR or 6.5% NaCl

Question 25

Strep ID flow alpha hemo

Answer 25

1. Optochin P disk 2. bile esculin 3. PYR bacitracin 6.5% NaCl

Question 26

Strep ID flow gamma hemo

Answer 26

1. CAMP or hippurate 2. bile esculin 3. PYR or bacitracin or 6.5% NaCl

Question 27

SXT trimethoprim-sulfmethoxazole disk resistance

Answer 27

GAS/GBS resistant

Question 28

PYR test

Answer 28

detects pyrrolidonyl arylamidase

Question 29

PYR reagent

Answer 29

N,N dimethylaminocinnamaldehyde

Question 30

GAS pyogenes treatment

Answer 30

penicillin or erythromycin

Question 30

GAS pyogenes treatment

Answer 30

penicillin or erythromycin

Question 31

GBS media

Answer 31

Todd-Hewitt, Trans-Vag broth, LIM, carrot

Question 32

GBS media

Answer 32

Todd-Hewitt, Trans-Vag broth, LIM, carrot

Question 33

CAMP test

Answer 33

streak perpendicular to S. aureus, beta lysin + CAMP factor = arrow head hemo, presumptive agalactiae

Question 34

Hippurate hydrolysis test

Answer 34

GBS, Sodium benzoate precipitate with ferric Cl, glycine purple with ninhydrin

Question 35

GBS agalactiae treatment

Answer 35

penicillin G, clindamycin, vancomycin

Question 36

Group C, F G strep ID

Answer 36

Beta hemo, use serogrouping

Question 37

pneumo morphology

Answer 37

diplococci, alpha hemo, autolyse

Question 38

pneumo virulence factors (3)

Answer 38

capsule IgA protease pneumolysin

Question 39

pneumolysin

Answer 39

similar to streptolysin O

Question 40

bile solubility test

Answer 40

Na deoxycholate accelerates autolysis, clear tube = pos

Question 41

enterococcus morphology

Answer 41

small, smooth, grey-blue colonies, alpha or gamma hemo

Question 42

bile esculin test

Answer 42

ability to grow in 40% bile and hydrolyze esculin, black precipitate pos

Question 43

6.5% salt tolerance test

Answer 43

pH indicator yellow pos enterococcus, negative purple/clear

Question 44

Viridans strep ID

Answer 44

catalase neg, optochin resistant, bile esculin neg, not bile soluble, no capsule

Question 45

satelliting strep abiotrophia and granulicatella are ___ deficient

Answer 45

B6 deficient, can grow with S. aureus