Final Exam Flashcards
Determine what it means for a plant to be considered “transgenic”.
A plant containing recombinant DNA. An organism that has been genetically modified.
Plants in which DNA from another species has been integrated into the host plant genome.
Identify the two major plant transformation methods, including benefits and drawbacks of each.
Agrobacterium tumefaciens: a soil bacterium inserts a fragment of its own DNA into plant cells.
Induces those cells to produce the plant hormones auxin and cytokinin and divide.
Cells form a tumor-like growth called a crown gall tumor.
Benefits: removes most of the genes that induce tumor formation, maintaining genes that insert DNA into plant chromosomes. Insert DNA that encodes for desirable traits.
Drawbacks: not effective in monocots.
Gene gun: DNA is coated on gold particles and forced inside plant cells with gas pressure.
Benefits: Much faster
Drawbacks: more expensive and not as precise
Be able to describe the two major transgenic technologies that have been adopted in US agriculture (as well as the plants in which they have been most widely adopted), and identify at least two other types of transgenic technologies that have been developed and/or are in use.
Insect Resistance: Genes from the Cry gene family of the bacteria bacillus thuringiensis (Bt), which encode for insecticidal toxin. Insects ingest protein and starve. Cotton and corn.
Herbicide tolerance: Engineer crop plants with an enzyme that will make them resistant to herbicides so weeds die while engineered plants survive. Round up ready soy beans.
Others: Fungal resistance, Cold tolerance, Added nutritional value
Describe what plants are used as biofuel sources, including which plant polysaccharides are used, and why.
Switchgrass: 5x more energy than required to be grown, harvested and processed into ethanol. Miscanthus: grows fast, low nutrient requirements, produces large yields, C4 plant. Corn (starch)-> glucose -> ethanol by fermentation Corn stover (cellulose) -> glucose -> ethanol by fermentation Others: Sweetpotato, Arundo donax
Explain why sequencing plant genomes is important.
So that we can learn and compare genes/sequences and use this knowledge to our advantage in plant biotechnology.
Explain, generally, how plant hormones were initially discovered.
Experiment by Charles Darwin showed that growth was dependent on light being perceived by tip of a growing seed shoot. Covered up the tips of seedling shoots and removed them, plants did not grow towards light.
Boysen-Jensen determined that a chemical at the tips was responsible.
Went put tips in agar (gelatin), then put the agar block on the cut end of the seedling.
Explain how plants respond to a gravity vector, taking into account the effects of auxin on the response.
Negative gravitropic: Auxins low at top of stem coming from right of seed, high at bottom of stem, to make it curve right and upward.
Positive gravitropic: Auxins low at top of root coming from left of seed, high at bottom because they get too high and inhibit growth, root growing left and downward.
Distinguish between short-day, long-day, intermediate and day-neutral plants with respect to critical day length and night length requirements, and when during the year each group is more likely to flower.
Long-day plants (short nights): Plants will not flower unless the day length is longer than critical period. Late spring/summer/early fall. Wheat, spinach, potatoes, oats.
Short-day plants (long nights): Plants that will not flower unless the day length is shorter than a critical period. Late fall/winter/very early spring. Duck weed, mums, poinsettias.
Intermediate: (transitional). No flowering if the daylength is too long or too short. Grasses.
Day-neutral: Flower irrespective of daylength. Resources influence flowering time. Tropicals, roses, tomatoes.
Be able to define turgor pressure, and give examples of plant movements that result from localized shifts in turgor.
Turgor pressure is pressure within a cell resulting from the uptake of water.
Turgor movement examples are Venus Fly Trap, Mimosa leaflets, Morning glory flowers opening and closing.
Be able to explain terms introduced in the ecology lecture (including the concept and definition of a biological community), as well as specific ways that humans contribute to loss of plant biodiversity.
Ecology: study of organismal interactions with one another and environment.
Population/Community/Landscape (Ecosystem)
Biological community: Multiple species living together that have the potential to physically and chemically interact.
Properties of communities: Diversity, Characteristic species, Stability, Exibit trophic structure (feeding relationships, energy transfer).
Be able to identify richness, density, and relative abundance of species if given a sample of community data, as we discussed in class.
Richness: number of different species
Density: number of individuals of a species
Relative abundance: number of organisms/individuals per unit area compared with the rest of them.
Diversity: high richness with high density
Explain what roles of plants and algae as producers in community and ecosystems.
a
Define primary productivity and identify types of ecosystems that have high and low primary productivity.
a
Distinguish between the criteria for plants to be considered threatened, endangered, and invasive.
Threatened: Likely to become endangered in a large portion of its natural growth rate.
Endangered: In danger of becoming extinct through all or a significant portion of its known growth range.
Be able to distinguish between primary and secondary succession if give examples of each, and types of disturbances.
a
What charge, if any, does DNA have?
negative
Agarose is extracted from ___________.
seaweed
The technique we used to separate our DNA is called?
Gel electrophoresis
The ____________ stain adheres to the DNA fragments which will fluoresce under UV light.
Gel Red
What is an enzyme capable of synthesizing new DNA strands that is isolated from the heat-loving bacterium Thermus aquaticus?
Taq Polymerase
What do you call the DNA which we tested for transgenic sequences that we isolated from plants and food products in our lab?
Template DNA
In DNA Adenine, Guanine, Cytosine, and Thymine are the “building blocks” that make the rungs of the double helix ladder. What is the name of the group that these four molecules belong in called?
nucleotides
What is the difference between vein pattern in monocots and dicots?
Monocots: parallel/linear
Dicots: branched/netted reticulate
Are palisade mesophyll present in monocots, dicots or both?
just Dicots
Stomata primarily located on which surfaces in monocots and dicots?
Monocots: both
Dicots: lower epidermis
What are the vascular bundle arrangements for monocots and dicots?
Monocots: scattered
Dicots: ring
Is there a pith present in monocots, dicots or both?
just Dicots
Is there a vascular cambium and cork cambium present in monocots, dicots or both?
just Dicots
What is the root system structure of monocots and dicots?
Monocots: fibrous
Dicots: taproot
Is there a pith present in the roots of monocots, dicots, or both?
just monocots (roots only)
What are the floral organs usually in multiples of monocots and dicots?
Monocots: 3’s
Dicots: 4’s and 5’s
Be able to draw a simple plant cell illustrating and labeling the locations of the plasma membrane, primary cell wall and secondary cell wall.
Plasma membrane: inside line
Secondary cell wall: shaded inner part between the 2 lines
Primary cell wall: outside line
The primary cell wall is principally composed of what polysaccharide?
?
__________ is the component of the secondary cell wall that gives it its hardness/toughness.
Lignin
Basic units are amino acids, of which there are 20 different types. (Does this describe lipids, carbohydrates, nucleic acids or proteins)
Proteins
Most caloric content per gram, as experimentally determined in lab. (Does this describe lipids, carbohydrates, nucleic acids or proteins)
Lipids
Fructose and sucrose are examples. (Does this describe lipids, carbohydrates, nucleic acids or proteins)
Carbohydrates
Primary elemental composition is CHONP; DNA & RNA. (Does this describe lipids, carbohydrates, nucleic acids or proteins)
Nucleic acids
Stained by Sudan IV, significant quantities in walnuts. (Does this describe lipids, carbohydrates, nucleic acids or proteins)
Lipids
Rubisco, PEP Carboxylase, and ATP Synthase are enzyme examples. (Does this describe lipids, carbohydrates, nucleic acids or proteins)
Protein
Stored in amyloplasts. (Does this describe lipids, carbohydrates, nucleic acids or proteins)
Carbohydrates
True or False:
Fatty acid chains of membrane lipid molecules can be considered unsaturated as long as they have at least one double-bond between two adjacent carbon atoms, creating a bend in the chain’s structure.
True
True or False:
The plasma membrane of plant cells is composed of a combination of lipids, proteins and carbohydrates.
True
Pinophyta, Magnoliophyta, and Bryophyta are titles used to name three very different groups of plants at what level of taxonomic classification?
?
Monocot and dicot angiosperms are separated at what other level taxonomic classification?
?
True or False:
Angiosperms are all grouped into one phylum.
True
Give an example of a monocot and dicot plant or plant family.
Monocot: rice
Dicot: Fabaceae
Briefly summarize some of the historical origins of botany.
?
List any 2 elements that are macronutrients.
?
List any 3 elements that are micronutrients.
Mo, Zn, Cu
Why are micronutrients called by that name?
smaller amounts are needed for nutrition
The vast majority of a plant’s mass can be accounted for by 3 principal structural elements. What are these elements and from what 2 major sources are they derived?
3 elements: C, O, H
2 sources:
CO2 and H2O
The light reactions take place in and across the membranes of structures called _____________.
Thylakoids
These structures are grouped together as grana within ____________________.
Chloroplasts
The primary photosynthetic pigment in chloroplasts is ____________________.
?
A structurally related pigment, _____________, also absorbs light and transfers energy to the primary pigment.
?
Multiple pigment molecules cluster together to form _______________, each of which are composed of light harvesting complexes and a reaction center.
photosystems
_______________ ________________ are pigments that reflect excess light and protect the other photosynthetic pigments in plant cells.
?
___________________ are the principal type of these pigments and are typically orange in color.
Carotenoids
Photosynthesis is most active in what two color ranges of the visible light spectrum?
blue and red
In light reactions, photosystem II, _________ molecule split to reduce/stabilize this photosystem to produce _____________ as a byproduct.
H20
O2
In light reactions, between photosystem II and I, _______________ energy molecule produced as a result of chemical energy generated from electron transport.
ATP
What is produced from light reactions in Photosystem I (the 2nd photosystem)?
NADPH
List 2 factors that could negatively impact the efficiency of the light reactions. Be specific about the cause and effect relationship of these factors.
?
The ___________ ___________ takes place in the stroma of chloroplasts.
Calvin Cycle
The Calvin Cycle starts with what gas?
CO2
In the Calvin Cycle, how many 3-carbon compounds are required in each step?
?
6
1 and 5
What energy molecule is required for the Calvin Cycle?
ATP
What is the last step of the Calvin Cycle before it starts over again?
(3) ? (RuBP)
What does the Reduction phase of the Calvin Cycle consist of?
?
The phase/step of the Calvin Cycle where ATP is used towards the end is what?
?
True or False:
All plants have the Calvin Cycle.
False
The enzyme that facilitates the fixation of carbon and the synthesis of 3-carbon compounds in the Calvin cycle is ________________, which is also the most abundant protein on the planet.
Rubisco
Rubisco interacts with __________ to ultimately produce two 3-carbon compounds during _______________.
O2
Photorespiration
True or False:
Photorespiration is an energy efficient mechanism to put carbon into the Calvin cycle because it produces more ATP.
False
In carbon fixation, which strategy/strategies of CAM, C3, C4 apply to:
CO2 diffuses from the air into mesophyll cells and is directly fixed into the Calvin cycle by Rubisco.
C3
In carbon fixation, which strategy/strategies of CAM, C3, C4 apply to:
Guard cells open stomata at night only, when CO2 diffuses in and water loss is minimized.
CAM
In carbon fixation, which strategy/strategies of CAM, C3, C4 apply to:
CO2 is initially fixed into a 4-carbon compound.
CAM and C4
In carbon fixation, which strategy/strategies of CAM, C3, C4 apply to:
Potential for photorespiration is low to zero because CO2 is concentrated spatially in bundle sheath cells.
C4
In carbon fixation, which strategy/strategies of CAM, C3, C4 apply to:
Examples include orchids, pineapple and jade plants.
CAM
In carbon fixation, which strategy/strategies of CAM, C3, C4 apply to:
Most abundant in terms of percentage of plant species diversity.
C3
In carbon fixation, which strategy/strategies of CAM, C3, C4 apply to:
Most likely to undergo photorespiration during drought or heat stress.
C3
In carbon fixation, which strategy/strategies of CAM, C3, C4 apply to:
Plants with this fixation mechanism are adapted to thrive in tropical or desert conditions.
CAM
The metabolic products of aerobic respiration are CO2 and water, but these reactions also result in a controlled release of energy in what 2 forms?
heat and ATP
Yeasts use fermentation to generate ATP molecules when oxygen is limited or not available. During this process, plant sugars are often broken down and two byproducts are generated, what are they?
CO2 and Ethanol
Compared to aerobic respiration, the amount of ATP generated in fermentation is higher or lower?
lower
What are the six plants that compose 80% of the world’s daily caloric consumption?
- Sweetpotato
- Cassava
- Wheat
- Corn
- ?
- ?
Approximately how many living plant species have been identified?
about 350,000
List 2 plants from which oilseed production is important.
canola
olive
Which cell type(s) is/are the follow characteristic of (Parenchyma, Collenchyma, Sclerenchyma)?
Have a primary and secondary cell wall.
Sclerenchyma
Which cell type(s) is/are the follow characteristic of (Parenchyma, Collenchyma, Sclerenchyma)?
Mostly isodiametric in shape.
? Parenchyma?
Which cell type(s) is/are the follow characteristic of (Parenchyma, Collenchyma, Sclerenchyma)?
Means “hard”.
Sclerenchyma
Which cell type(s) is/are the follow characteristic of (Parenchyma, Collenchyma, Sclerenchyma)?
Primary wall is unevenly thickened for structural reinforcement.
Collenchyma
Which cell type(s) is/are the follow characteristic of (Parenchyma, Collenchyma, Sclerenchyma)?
Cells found in meristems / mother of all cell types.
Parenchyma
Which cell type(s) is/are the follow characteristic of (Parenchyma, Collenchyma, Sclerenchyma)?
Fibers are these.
Sclerenchyma
Which cell type(s) is/are the follow characteristic of (Parenchyma, Collenchyma, Sclerenchyma)?
Living at maturity.
Parenchyma, Collenchyma
Rank the following magnification instruments in order of their magnification strengths lowest to highest: Hand lens, Compound microscope, Stereomicroscope
- Hand lens
- Stereomicroscope
- Compound microscope
Which microscope would you use to observe cellular structure at high resolution?
Compound microscope
Determine whether the following example or statement is descriptive of Alkaloid, Phenolic, or Terpene specialized metabolites:
Many fragrent essential oils, including those found in eucalyptus, thyme and mint.
Terpene
Determine whether the following example or statement is descriptive of Alkaloid, Phenolic, or Terpene specialized metabolites:
Include opiate sedatives and analgesics: morphine, codeine, etc.
Alkaloid
Determine whether the following example or statement is descriptive of Alkaloid, Phenolic, or Terpene specialized metabolites:
Lignin; aspirin precursor salicylic acid from willow tree bark.
Phenolic
Determine whether the following example or statement is descriptive of Alkaloid, Phenolic, or Terpene specialized metabolites:
Includes carotenoids and xanthophylls.
Terpene
Determine whether the following example or statement is descriptive of Alkaloid, Phenolic, or Terpene specialized metabolites:
Urushiol, the dermatitis compound produced by poison ivy, as well as THC produced by Cannabis.
Phenolic
Determine whether the following example or statement is descriptive of Alkaloid, Phenolic, or Terpene specialized metabolites:
Natural rubber that is derived from the latex of Havea brasiliensis.
Terpene
Determine whether the following example or statement is descriptive of Alkaloid, Phenolic, or Terpene specialized metabolites:
Members include the powerful stimulant and anesthetic cocaine as well as deadly compounds derived from hemlock that inhibit motor neuron activity.
Alkaloid
Determine whether the following example or statement is descriptive of Alkaloid, Phenolic, or Terpene specialized metabolites:
Colorful compounds including red and purple anthocyanins observed in flowers and fruits; attract potential pollinators and see dispersers.
Phenolic
Determine whether the following example or statement is descriptive of Alkaloid, Phenolic, or Terpene specialized metabolites:
Bitter compounds in the white “pith” of many citrus fruits; strong insect deterrents.
Terpene
The___________ theory states that two specific plant organelles may in fact be the result of an ancient symbiosis between a host cell and two different types of bacteria. What is one of those organelles?
endosymbiotic theory
mitochondria
Which statements are used to support the endosymbiotic theory out of the following:
- They connect living plant cells to each other
- They have their own DNA genomes
- They have highly specialized metabolic activities (ie. photosynthesis and respiration)
- They have their own primary cell walls
- They have their own DNA genomes
3. They have highly specialized metabolic activities (ie. photosynthesis and respiration)
Vocab:
Minute strands of cytoplasm that extend between adjacent cells through pores in the walls.
Plasmodesmata
Vocab:
A layer of material, rich in pectin, that cements two adjacent cell walls together.
Middle lamella
Vocab:
Round or elliptical thin area in a cell wall occurring in pairs opposite each other, with or without shallow, domelike borders.
Pits
Vocab:
A pocket of fluid that is separated from the cytoplasm of a cell by membrane. Also, food-storage or contractile pockets within the cytoplasm of unicellular organisms.
Vacuole
Vocab:
Plastids containing pigments other than chlorophyll, usually yellow to orange.
Chromoplasts
Vocab:
A pair of specialized cells surrounding a stoma.
Guard cells
Plants possess a number of different types of crystals, many of which are thought to thwart predators from continuous feeding. What chemical compound are these crystals composed?
?
What is the carbohydrate that is most commonly transported long distances in plants?
?
The carbohydrate that is most commonly transported long distances in plants is a mono-, di-, or poly- saccharide?
disaccharide
Vocab:
A primary meristematic tissue that differentiates into primary xylem and phloem.
Procambium
Vocab:
Zone of Differentiation
Vocab:
Zone of Cell Division
Vocab:
A meristem at the tip of a shoot or root.
Apical Meristem
Vocab:
A thimble-shaped mass of cells at the tip of a growing root; functions primarily in protection.
Root Cap