Final Exam Flashcards
(309 cards)
Determine what it means for a plant to be considered “transgenic”.
A plant containing recombinant DNA. An organism that has been genetically modified.
Plants in which DNA from another species has been integrated into the host plant genome.
Identify the two major plant transformation methods, including benefits and drawbacks of each.
Agrobacterium tumefaciens: a soil bacterium inserts a fragment of its own DNA into plant cells.
Induces those cells to produce the plant hormones auxin and cytokinin and divide.
Cells form a tumor-like growth called a crown gall tumor.
Benefits: removes most of the genes that induce tumor formation, maintaining genes that insert DNA into plant chromosomes. Insert DNA that encodes for desirable traits.
Drawbacks: not effective in monocots.
Gene gun: DNA is coated on gold particles and forced inside plant cells with gas pressure.
Benefits: Much faster
Drawbacks: more expensive and not as precise
Be able to describe the two major transgenic technologies that have been adopted in US agriculture (as well as the plants in which they have been most widely adopted), and identify at least two other types of transgenic technologies that have been developed and/or are in use.
Insect Resistance: Genes from the Cry gene family of the bacteria bacillus thuringiensis (Bt), which encode for insecticidal toxin. Insects ingest protein and starve. Cotton and corn.
Herbicide tolerance: Engineer crop plants with an enzyme that will make them resistant to herbicides so weeds die while engineered plants survive. Round up ready soy beans.
Others: Fungal resistance, Cold tolerance, Added nutritional value
Describe what plants are used as biofuel sources, including which plant polysaccharides are used, and why.
Switchgrass: 5x more energy than required to be grown, harvested and processed into ethanol. Miscanthus: grows fast, low nutrient requirements, produces large yields, C4 plant. Corn (starch)-> glucose -> ethanol by fermentation Corn stover (cellulose) -> glucose -> ethanol by fermentation Others: Sweetpotato, Arundo donax
Explain why sequencing plant genomes is important.
So that we can learn and compare genes/sequences and use this knowledge to our advantage in plant biotechnology.
Explain, generally, how plant hormones were initially discovered.
Experiment by Charles Darwin showed that growth was dependent on light being perceived by tip of a growing seed shoot. Covered up the tips of seedling shoots and removed them, plants did not grow towards light.
Boysen-Jensen determined that a chemical at the tips was responsible.
Went put tips in agar (gelatin), then put the agar block on the cut end of the seedling.
Explain how plants respond to a gravity vector, taking into account the effects of auxin on the response.
Negative gravitropic: Auxins low at top of stem coming from right of seed, high at bottom of stem, to make it curve right and upward.
Positive gravitropic: Auxins low at top of root coming from left of seed, high at bottom because they get too high and inhibit growth, root growing left and downward.
Distinguish between short-day, long-day, intermediate and day-neutral plants with respect to critical day length and night length requirements, and when during the year each group is more likely to flower.
Long-day plants (short nights): Plants will not flower unless the day length is longer than critical period. Late spring/summer/early fall. Wheat, spinach, potatoes, oats.
Short-day plants (long nights): Plants that will not flower unless the day length is shorter than a critical period. Late fall/winter/very early spring. Duck weed, mums, poinsettias.
Intermediate: (transitional). No flowering if the daylength is too long or too short. Grasses.
Day-neutral: Flower irrespective of daylength. Resources influence flowering time. Tropicals, roses, tomatoes.
Be able to define turgor pressure, and give examples of plant movements that result from localized shifts in turgor.
Turgor pressure is pressure within a cell resulting from the uptake of water.
Turgor movement examples are Venus Fly Trap, Mimosa leaflets, Morning glory flowers opening and closing.
Be able to explain terms introduced in the ecology lecture (including the concept and definition of a biological community), as well as specific ways that humans contribute to loss of plant biodiversity.
Ecology: study of organismal interactions with one another and environment.
Population/Community/Landscape (Ecosystem)
Biological community: Multiple species living together that have the potential to physically and chemically interact.
Properties of communities: Diversity, Characteristic species, Stability, Exibit trophic structure (feeding relationships, energy transfer).
Be able to identify richness, density, and relative abundance of species if given a sample of community data, as we discussed in class.
Richness: number of different species
Density: number of individuals of a species
Relative abundance: number of organisms/individuals per unit area compared with the rest of them.
Diversity: high richness with high density
Explain what roles of plants and algae as producers in community and ecosystems.
a
Define primary productivity and identify types of ecosystems that have high and low primary productivity.
a
Distinguish between the criteria for plants to be considered threatened, endangered, and invasive.
Threatened: Likely to become endangered in a large portion of its natural growth rate.
Endangered: In danger of becoming extinct through all or a significant portion of its known growth range.
Be able to distinguish between primary and secondary succession if give examples of each, and types of disturbances.
a
What charge, if any, does DNA have?
negative
Agarose is extracted from ___________.
seaweed
The technique we used to separate our DNA is called?
Gel electrophoresis
The ____________ stain adheres to the DNA fragments which will fluoresce under UV light.
Gel Red
What is an enzyme capable of synthesizing new DNA strands that is isolated from the heat-loving bacterium Thermus aquaticus?
Taq Polymerase
What do you call the DNA which we tested for transgenic sequences that we isolated from plants and food products in our lab?
Template DNA
In DNA Adenine, Guanine, Cytosine, and Thymine are the “building blocks” that make the rungs of the double helix ladder. What is the name of the group that these four molecules belong in called?
nucleotides
What is the difference between vein pattern in monocots and dicots?
Monocots: parallel/linear
Dicots: branched/netted reticulate
Are palisade mesophyll present in monocots, dicots or both?
just Dicots