Fixatives

Question 1

B-5

Answer 1

1. Bone marrow, lymph nodes, spleen (hematopoietic & lymphoreticular tissue)
2. Now B+ fixative is B5 substitute without the HgCl2

Question 2

Bouin

Answer 2

1. Good for GI biopsies
2. Connective tissue staining
3. Fixation takes 6h
4. Transferred to 70% alcohol prior to remove picrates.
   -lyses RBC’s
   -decalcified some amounts of calcium

Question 3

Gendre

Answer 3

Glycogen

Question 4

Holland

Answer 4

1. Gastrointestinal
2. Stabilize RBC

Question 5

Zenker

Answer 5

1. Good for Trichome stains
2. Bloody specimens & bone marrow biopsies
3. Fixed tissue washed overnight in running tap water before processing to remove chromate
4. Fixation time 4-24h

Question 6

Helly

Answer 6

1. Bone marrow aspirate (bloody)
2. Fixation time 12-24h
3. Good water wash to remove chromate

Question 7

Carnoy’s

Answer 7

1. RNA stains, cytology for bloody specimen, glycogen preservation
2. No water
3. Fixation time 1-3h
4. Fixed tissue processed immediately or transferred to 80% alcohol