FMB - Lab Lecture 1

Question 1

Used for handling, isolating, transferring, and cultivating microbes.

Answer 1

Inoculating Loop

Question 2

Used for stabbing bacteria vertically into deep tube of media to determine the shape of the hole digested by the bacteria.

Answer 2

Inoculating Needle

Question 3

Used for spreading the bacteria in the plate.

Answer 3

L-shape glass rod

Question 4

Can be used as spreader and can also be used during sampling instead of inoculating loops.

Answer 4

Cotton Swab

Question 5

Allow the users to extract or deliver small amount of liquid of microbial dilutions.

• micro liter
• “exact quantity”

Answer 5

Mechanical pipettes

Question 6

Used in heating substances and in incineration the specialized tool to avoid contaminants.

Answer 6

Alcohol Lamp

Question 7

Used for the cultivation of microorganism on solid media.

Answer 7

Petri plates

Question 8

Can be used for culturing, maintaining and storing cultures, specifically in broth.

Answer 8

Test Tubes

Question 9

Important in microbial dilutions for microbial count.

Answer 9

Test Tubes

Question 10

Hold the microbial cultures and store liquids.

Answer 10

Erlenmeyer Flask

Question 11

Can also be used for the preparation of culture media.

Answer 11

Erlenmeyer Flask

Question 12

Hold and work in mixing culture media and other liquids.

Answer 12

Beaker

Question 13

Not recommended in culturing bacteria due to its open space or wide mouth.

Answer 13

Beaker

Question 14

Latin word of Microscope

Answer 14

Microscopium

Question 15

“to look at”

Answer 15

skopein

Question 16

A laboratory instrument used to examine objects that are to small to be seen by the naked eye.

Answer 16

Microscope

Question 17

Contains more than one magnifying lens.

Answer 17

Compound Light Microscope

Question 18

The image from the _________ is magnified again by the __________ .

Answer 18

1. Objective Lens
2. Ocular Lens

Question 19

Maximum magnifying power of about ________.

Answer 19

1,000 x

Question 20

Specimen remain in focus.

Answer 20

Parfocal

Question 21

Remagnifies the image formed by the objective lens.

Answer 21

Ocular lens

Question 22

Transmit the image from the objective lens to the ocular lens.

Answer 22

Body tube

Question 23

Primary lenses that magnify the specimen.

Answer 23

Objective lenses

Question 24

Holds the microscope slide in position.

Answer 24

Stage

Question 25

Focuses light through the specimen.

Answer 25

Condenser

Question 26

Controls the amount of light entering the condenser.

Answer 26

Diaphragm

Question 27

Light source.

Answer 27

Illuminator

Question 28

A device that uses steam to sterilize equipment and other objects. This means that all bacteria, viruses, fungi, and spores are inactivated.

Answer 28

Autoclave

Question 29

It is a pressurized device that enables the heating of aqueous solutions up to temperatures above the boiling point of water.

Answer 29

Autoclave

Question 30

It works by allowing steam to enter and maintaining pressure of ________ . This causes the steam to reach _________ for ________.

Answer 30

1. 15 psi 2. 121 degree C 3. 15 Minutes

Question 31

When the holding period is over, the heater is turned off and the autoclave allowed to cool till the pressure inside is equal to the atmospheric pressure.

Answer 31

Autoclave

Question 32

Provide a high degree of localized control for critical processes.

Answer 32

Laminar flow hood

Question 33

The work area is continuously bathed with positive pressure HEPA- filtered air to protect the product from contamination.

Answer 33

Laminar flow hood

Question 34

Designed for the handling of materials in a sterile working environment.

Answer 34

Laminar flow hood

Question 35

A device used to grow and maintain microbiological cultures or cell cultures.

Answer 35

Incubator

Question 36

Maintains optimal temperature, humidity and other conditions such as the carbon dioxide (CO2) and oxygen content of the atmosphere inside.

Answer 36

Incubator

Question 37

Highly sensitive lab instruments designed to accurately measure mass.

Answer 37

Analytical Balance

Question 38

* have finer readability, * more sensitive to changes, and * can detect smaller variations in mass

Answer 38

Analytical Balance

Question 39

Can safely be used in microbiology laboratory for preparation of media.

Answer 39

Microwave

Question 40

It may be used to preserve certain chemical reagents or media.

Answer 40

Refrigerator

Question 41

It may be used to grow certain types of bacteria.

Answer 41

Refrigerator

Question 42

This can also be used to preserve cultures of bacteria, yeast, and other microbes.

Answer 42

Refrigerator

Question 43

Cooked cut potato - earliest solid medium

Answer 43

Robert Koch

Question 44

* not satisfactory due to low melting point * liquefy at 24 degree C

Answer 44

Gelatin

Question 45

Agar

Answer 45

Frau Hesse

Question 46

* Most used to prepare solid media * Polysaccharide extract obtained from seaweed. * No nutritive value * Not affected by the growth of the bacteria.

Answer 46

Agar

Question 47

Melts at ___ to ___ & sets at ___ to ____ .

Answer 47

Melts at 98 to 100 degree C & sets at 42 to 45 degree C.

Question 48

_____ agar is employed in solid medium

Answer 48

2%

Question 49

AGAR: AN IDEAL SOLIDIFYING AGENT

Answer 49

(a) Bacteriologically inert. (b) No influence on bacterial growth. (c) It remains solid at 37°C. (d) It is transparent.

Question 50

Exact composition is not known.

Answer 50

Empirical or Natural

Question 51

Have known quantities of all ingredients.

Answer 51

Synthetic or Defined

Question 52

Empirical or Natural examples.

Answer 52

milk, urine, diluted blood, vegetable juices, meat extracts, infusions and peptone.

Question 53

Synthetic or Defined

Answer 53

Glucose or glycerol are often used as carbon sources, and ammonium salts or nitrates as inorganic nitrogen sources.

Question 54

Liquid (broth)

Answer 54

* used for profuse growth * Mixed organisms cannot be separated.

Question 55

Solid (slant, plate)

Answer 55

* Bacteria may be identified by studying the colony character. * Mixed bacteria can be separated. * Solid media is used for the isolation of bacteria as pure culture.

Question 56

Semi-solid (soft agar)

Answer 56

* Contains 0.5% agar * Usually for motility media * Isolation of bacteriophage

Question 57

May be used for growth (culture) of bacteria that do not need enrichment of the media.

Answer 57

General or Non-selective

Question 58

General or Non-selective example.

Answer 58

Enterobacteriaceae and Staphylococcus spp.

Question 59

Macroscopically visible collection of millions of bacteria originating from a single bacterial cell.

Answer 59

Colony

Question 60

Favors the growth of a particular bacterium by inhibiting the growth of undesired bacteria and allowing growth of desirable bacteria.

Answer 60

Selective media

Question 61

uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators added to the medium to visibly indicate the defining characteristics of a microorganism.

Answer 61

Differential

Question 62

Lactose Fermenting Colonies

Answer 62

Pink

Question 63

Non-lactose Fermenting Colonies

Answer 63

Colorless

Question 64

MacConkey's Agar

Answer 64

Gram negative bacteria

Question 65

Macrospically visible collection of millions of bacteria originating from a single bacterial cell. 25-200 CFU's

Answer 65

Colony

Question 66

Mechanical Parts of Microscope:

Answer 66

- Base - Pillar - Arm - Stage - Inclination Joint - Stage clips - Revolving nose piece - Body Tube - Draw Tube - Fine ajdustment knob - Course adjustment knob

Question 67

Optical Parts of Microscope:

Answer 67

- Objective Lens - Ocular Lens

Question 68

Illuminating Parts of Microscope:

Answer 68

- Diaphragm - Mirror