Fungi 3

Question 1

How does C.albicans adhere to host membranes?

Answer 1

Cell wall proteins (7 adhesins- ALS1–>7)
GPI, Eap1 and Hwp

Question 2

What is GPI in fungal adherence?

Answer 2

glycosylphosphatidylinositol

Question 3

What is Eap1 in fungal adherence?

Answer 3

Enhanced Adherence to Polystyrene

Question 4

What is Hwp1 in fungal adherence?

Answer 4

Hyphal Wall Protein

Question 5

How does yeast initially bind to host cells?

Answer 5

Via the use of Als1 and Eap1

Question 6

How do hyphae bind to host cells and promote biofilm formation?

Answer 6

Via the use of Als3 an Hwp1

Question 7

Stages of fungal biofilm formation?

Answer 7

Adherence, initiation, maturation, dispersal

Question 8

Benefit of fungi forming biofilms?

Answer 8

Resistance–> if one of the fungi in the biofilm is resistant to smthn it can help provide this to the rest of the biofilm

Question 9

Two routes that C.albicans can invade host tissues?

Answer 9

Induced endocytosis, Active penetration

Question 10

How does induced endocytosis work?

Answer 10

Invasins promote host binding to cadherin receptors - induce endocytosis

Question 11

How does active penetration?

Answer 11

Secreted hydrolase – damage membranes (also provide nutrition)