Genetic Linkage and Direct Testing Flashcards
What is Linkage ?
The tendency of genes and segments of DNA closely positioned along a strand of DNA to segregate together in meiosis and therefore be inherited together
Is linkage analysis direct or indirect analysis ?
Indirect DNA analysis
What is linkage analysis ?
The use of several DNA markers that are near or within a gene of interest to track within a family of inheritance of a disease causing mutation of that gene
What is a marker ?
An identifiable segment of DNA (SNP, satellite, VNTR, ) with enough variation between individuals that the inheritance and co-inheritance of alleles of a given gene can be traced
What is a phase ?
That for two or more closely linked markers the alleles are contained on the same chromosome
What is linkage disequilibium ?
In a population, co-occurance of specific alleles at a higher frequency than would be predicted by random chance; over-representation of specific haplotypes
What is haplotype analysis ?
Molecular genetic testing to identify a set of closely linked segments of DNA; used in linkage analysis or when a given traits is in linkage disequilibrium with a marker or set of markers
What is one way a cell can generate new haplotypes in the offspring ?
Through recombination
What kind of markers do you want for linkage analysis ?
Markers that are very tightly linked
How is linkage an indirect analysis ?
You are indirectly measuring a marker very close to the gene of interest. The closer the gene of interest is to the marker the more accurate the whole process is. This is useful when you are not sure exactly what the specific allele for the phenotype is
What is the accuracy of clinical linkage analysis based on ?
The accuracy of clinical diagnosis, The distance between the disease casing mutation and the markers, the informativeness of the genetic markers
What kind of haplotypes are best for linkage analysis ?
Haplotypes that are very tightly linked because this will lower the chances of recombination
What are the three factors that determine the limitations of linkage analysis ?
- The accuracy of the clinical diagnosis
- The distance between the disease causing mutation and the genetic markers
- Informativeness of genetic markers
What is the utility of indirect genetic analysis ?
- When direct DNA analysis is not possible
2. When the disease causing mutation within the gene of interest is unknown in a specific family
What is the significance of the haplotype ? or Phase
It must be established in each family
What genetic tool can you use to determine if recombination has taken place?
Flanking markers which reside on each part of the chromosome of interest
Using Flanking sequences you can determine if a cross over has occured between your markers ? What implication will this have on your pedigree analysis ?
You will be unable to determine if the cross over was above or below the diseased gene and you wont be able to determine which gamete got the diseased gene.
What are the three categories of genetic testing ?
Infectious Disease Testing
Cancer Genetics
Molecular Genetics
What are common uses in linkage analysis ?
Muscular Dystrophy
Cystic Fibrosis
Inherited Cancer Syndrome
Any disease where the mode of inheritance is known.
What are two steps that are a must to do linkage analysis ?
You have to know the diagnosis and mode of inheritance
*You have to have multiple family members involved in the analysis
What is phase the same as ?
Haplotype
What are the molecular mechanisms of trinucleotide repeats ?
Inaccurate DNA replication and slippage when crossing over
What is direct testing good for ?
Determination of genotype for one or more specific mutations. Specific mutation in Favtor 5 Leiden Mutation, CF is less effective due to the fact that there are hundreds of mutations
What is the mechanism of inheritance for Fragile X ?
X linked, Expansion of the 5’ UTR that will result in a loss of function, accompanied with DNA methylation
