Growth Media

Question 1

media that allow different bacteria to be distinguished based on biochemical reactions, often by producing visible changes

Answer 1

differential

Question 2

media that contain specific agents that inhibit the growth of some bacteria while allowing the growth of others.

Answer 2

selective

Question 3

this media is selective for gram negative rods and differential for lactose fermenters and bile positive organisms

Answer 3

MacConkey

Question 4

this media is differential for hemolysis and enriched

Answer 4

5% sheep blood agar

Question 5

this media is selective for gram positive organisms and differential for hemolysis (added blood agar)

Answer 5

CNA (colistin and nalidixic acid)

Question 6

enriched heated blood media with hemin and NAD factors which are not selective or differential but grow fastidious bacteria

Answer 6

chocolate agar

Question 7

pre-reduced media to eliminate O2 and ensure the recovery of this type of bacteria

Answer 7

anaerobic media

Question 8

media used to select out an organism as well as differential by color the bacteria

Answer 8

chromogenic media

Question 9

media used for the Kirby-Bauer test

Answer 9

Mueller-Hinton media

Question 10

media for Mycoplasma and Chlamydia

Answer 10

SP4 media

Question 11

needs media that has antibiotics like the BCYE Selective Agar which contains anisomycin, colistin, and vancomycin

Answer 11

legionella species

Question 12

media to grow campylobacter jejuni which is specially formulated with antibiotics to reduce the growth of normal flora present (polymyxin B, trimethoprim, and vancomycin)

Answer 12

Campy BAP

Question 13

neisserria gonorrhea requires media with the presence of vancomycin, colistin, anisomycin (V-C-A Inhibitor) and trimethoprim, to suppress the normal flora

Answer 13

Martin Lewis Media

Question 14

selective and differential for MRSA

Answer 14

chromogenic media

Question 15

Fastidious GNR haemophillis species phenotypic characteristics (4)

Answer 15

-Nonmotile ​
-Aerobic to facultatively anaerobic​
-Most produce catalase and oxidase​
-Require either both or one of two factors for growth (hemin – X factor or NAD (nicotinamide adenine dinucleotide) – V factor) so they will not grow on Maconkey Agar and weakly to no growth on BAP Agar.​

Question 16

fastidious Small Gram-negative rod that is the cause of an upper respiratory infection called “whooping cough”.​

Answer 16

Bordetella species

Question 17

cause of systemic disease called brucellosis​ (fastidious GNR)

Answer 17

brucella species

Question 18

Cause of tularemia infection which can be systemic or respiratory (fastidious GNR)

Answer 18

francisella species

Question 19

most commonly encountered in the clinical microbiology laboratory that all grow on MacConkey

Answer 19

non fastidious aerobic Gram-negative rods

Question 20

distinguished from the other non fermenters by their absence of the production of the enzyme “oxidase”​

Answer 20

non lactose fermenters of the enterobacteriaciae group

Question 21

members of the Enterobacteriaceae are considered lactose positive​ (5)

Answer 21

Escherichia coli​

Klebsiella spp.​

Enterobacter spp.​

Serratia spp.​

Citrobacter spp.​

Question 22

Oxidase negative non-fermenters can be either of the following six Enterobacteriaceae:​

Answer 22

Morganella spp.​

Proteus spp.​

Providencia spp.​

Salmonella spp. – enteric pathogen​

Shigella spp. – enteric pathogen​

Yersinia spp. - plague​

Question 23

Gram negative bacilli most commonly isolated in human infections

Answer 23

Enterobacterales