Haematology Theory 💉 Flashcards

(43 cards)

1
Q

What are the common venepuncture sites for blood collection?

A
  • Jugular vein
  • Cephalic vein
  • Saphenous vein
  • Lateral ear vein (rabbits)
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2
Q

What gauge needle is commonly used for blood collection in most animals?

A

21-gauge (green) needle.

Notes…

Using the correct gauge is crucial to prevent damage to blood cells.

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3
Q

What is the risk of using a smaller needle, such as a 23-gauge (blue)?

A

It risks damaging the blood cells and could artificially alter the results.

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4
Q

What technique is employed to raise the vein for blood collection?

A

Occluding flow by applying pressure either by yourself or a colleague.

.

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5
Q

What must be added to blood samples if plasma is needed?

A

An anticoagulant must be added to stop the blood from clotting.

Notes…
Lithium heparin (orange or green)

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6
Q

What is the difference between blood samples for plasma and serum?

A

Plasma requires anticoagulant; serum is obtained without anticoagulant allowing clotting.

Understanding this distinction is vital for conducting various laboratory tests.

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7
Q

What must be added to blood samples if plasma is needed?

A

An anticoagulant must be added to stop the blood from clotting.

Anticoagulants are necessary for tests that require plasma.

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8
Q

What is the tube type for plain blood collection?

A

White / clear

Used for serum biochemistry tests.

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9
Q

What colour tube is lithium heparin?

A

Orange

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10
Q

What colour is a fluoride oxalate tube?

A

Yellow

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11
Q

What is the primary use of EDTA tubes?

A

Haematology

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12
Q

What effect does EDTA have on results?

A

Falsely elevates potassium concentrations and decreases calcium and magnesium concentrations.

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13
Q

What does EDTA bind, leading to false decreases in test results?

A

Calcium and magnesium

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14
Q

What are the two main types of modern haematology analysers?

A

Coulter counter and flow cytometer

Both are used for total blood counts.

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15
Q

What types of cells can haematology analysers differentiate?

A

Different types of blood cells

This helps in diagnosing various conditions.

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16
Q

What are the normal red blood cell counts for dogs?

A

5.5 - 8.5 x 10^12/1

This range is essential for assessing canine health.

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17
Q

What is the normal white blood cell count for cats?

A

5.5 - 19.5 x 10^9/

Monitoring WBC count is crucial for feline health.

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18
Q

Fill in the blank: EDTA stands for _______.

A

ethylene diamine tetra acetic acid

A common anticoagulant used in blood collection.

19
Q

What test is fluoride oxalate used for?

20
Q

What colour is a citrate tube and what is it used for?

A

Blue or pale green, clotting profiles

21
Q

What tubes can be used for serum blood biochemistry?

A

Plain (white) plain with gel (brown).

22
Q

What % of leukocytes are neutrophils?

23
Q

What % of leukocytes are eosinophils?

24
Q

What % of leukocytes are basophils?

25
What % of leukocytes are lymphocytes?
15-20%
26
What % of leukocytes are monocytes?
3-5%
27
What are the characteristics of a good blood smear?
-2/3 down the slide -tidy, parallel edges -feathered edge -monolayer
28
What problem is this?
Banding -caused by uneven pressure
29
What problem is this?
Striped effect -clots in the sample -dirt on the slide
30
What problem is this?
Spots in the smear -grease on the slide -fat in the blood
31
What problem is this?
Smear too narrow -insufficient time or blood
32
What are the types of romanowsky stain?
-leishman’s -wright’s -giemsa -Jenner’s -diffquik
33
What are the 3 agents used in diffquik?
1- methanol (fixer) 2- eosin 3- methylene blue
34
What are supra-vital stains?
Stains that must be cultured with the blood cells.
35
What temperature are supra-vital cells incubated at?
37 degrees Celsius
36
What are examples of supra-vital stains?
New methylene blue Brilliant cresyl blue
37
When are Heinz bodies seen?
Paracetamol toxicity in cats Onion/garlic toxicity in cats and dogs
38
What is the battlement technique?
Most commonly used technique for performing a differential count. 100 white blood cells must be counted and their types determined.
39
What are bands?
Immature neutrophils (they have white/clear granules and their nucleus is not yet lobed).
40
What is this a diagram of?
Crenation (crinkled red blood cells) due to dehydration or too much anti-coagulant.
41
What speed do you spin a PCV at?
10,000 rpm for 5min
42
What are the normal ranges for a dog PCV?
0.37-0.55 (37-55%)
43
What are the normal ranges for a cat PCV?
0.24-0.45 (24-45%)