Haemophilus and Pertussis Flashcards Preview

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Flashcards in Haemophilus and Pertussis Deck (35):
1

haemophilus morphology

gram neg rod
pleomorphic
non motile

2

haemophilus is associated with what other disease?

influenza

3

typeable vs nontypeable

encapsulated vs non encapsulated

4

what types of disease does encapsulated haemophilus cause?

respiratory infections and bacterial meningitis in kids

sinusitis

ear aches

can progress to:

bacteremia, meningitis, epiglotitits, cellulitis, or joint infections

before Hib vaccine, leading cause of bacterial meningitis in kids

5

what types of disease does unencapsulated haemophilus cause?

respiratory infections

ear aches

sinusitis

desseminate disease in compromised individual

no meningitis

6

what are the haemophilus capsule made of

carbohydrates

a-f are important, b is the worst

helps avoid immune system- antiphagocytic and anti-complement

7

who carries haemophilus

everyone- 75%

8

where does haemophilus live?

nose, spreads via aerosolied form

9

is there a haemophilus vaccine?

yes- to the b encapsulated form

vaccine was originally to capsule, more recently conjugated to protein

10

encapsulated Hi pathogensis

no exotoxins

endotoxins covered by capsule, so only contributes in bacteremia

Hib- LPS covered with choline

11

encapsulated Hi immunity

passive from mother for a few months

children susceptible from 6-12 months

acquire active immunity at about 3-4 years of age

12

treatment of Hib infection

for meningitis- 3rd gen cephlosporin or augmentin

low mortality with early treatment

prophylactic rifampin for contacts

13

nontypeable Hi pathogenesis

causes respiratory and ear infections, conjuctivitis

colonization starts in nasopharynx

kids get ear infections thru shorter eustachian tubes

only causes meningitis in compromised patients

14

HI adhesins

adhesins found in both types of Hi promote colonization'

so much variability that they arent good vaccine targets

15

is nonencapsulated Hi intra or extra cellular?

both

16

vaccines for unencapsulate Hi?

no

17

treatment for NTHI

treatment for otitis media- amoxicillin (resistant strain has b-lactamase inhibitor added)

18

Hi growth and metabolism

facultative anaerobe

no diagnostic fermentation

fragile and fastidious

doesnt grow on blood agar- uses chocolate agar

needs X factor- hemin, and v factor- NAD

these growth properties are used to identify

19

diagnosis of Hib

history/age

culture and blood/csf

presence of type b Ag

20

bordetella pertussis causes what?

whooping cough

21

bordetella pertussis morphology

gram negative coccobacillus

22

bordetella pertussis type of metabolism

obligate aerobe``

23

bordetella pertussis transmission

aerosol droplets

24

bordetella pertussis incubation period

7-10 days

25

whoop is caused by what?

strong inhalation following cough through a narrowed glottis

26

what age group is hit worst by bordetella pertussis?

infants

27

is there bacteremia in bordetella pertussis

no

28

exotoxins in bordetella pertussis

pertussis toxin- increases cAMP
causes: effects innate immunity, increases insulin, sensitization to histamine

adenylate cyclase toxin- increases cAMP

dermonecrotic toxin- interacts w/ actin polymerization
causes local necrosis and inflammation

29

other toxins frombordetella pertussis

tracheal cytotoxin- peptidoglycan stops cilia from beating and kills cells. causes inflammtion

LPS- activates innate immune system

30

bordetella pertussis adhesive virulence factors

pili- mediates attachment to respiratory tract

Fha- binds galactose

pertactin- outer membrane molecule

tracheal colonization factor- outer membrane molecule

31

bordetella pertussis vaccine

subunit vaccine

pertussis toxoid, fha, pertactin all part of vaccine

part of DTaP

32

reasons for increase in bordetella pertussis disease

waning immunity, Ag divergence, mutation of PTX, or decreased vaccination

33

diagnosis of bordetella pertussis

cough

isolation of organism

lymphocytosis

history of contacts

34

detection of bordetella pertussis

must be cultured of bordet gengou agar- special

PCR

35

bordetella pertussis treatment

Abs and hypoxia management