HEMA LAB MOD 2

Question 1

Numerical evaluation of the formed elements (RBC, WBC, and platelets) in the blood

Answer 1

Hemocytometry

Question 2

___ means blood

___ cell

Answer 2

hemo

cyto

Question 3

2 methods in hemocytometry

Answer 3

turbidimetric method

microscopic method

Question 4

___ method is no longer used because of its inaccuracy and imprecision

Answer 4

turbidimetric method

Question 5

In this method, there are solutions with known number of cells and the unknown is compared with the solutions

Answer 5

Turbidimetric method

Question 6

___ method uses a microscope to count the cells.

Answer 6

Microscopic method

Question 7

____ is the estimation of a number of blood cells in a known volume of blood

Answer 7

Microscopic hemocytometry

Question 8

another name for hemocytometer

Answer 8

counting chamber

Question 9

another name for hemocytometer

Answer 9

counting chamber

Question 10

The principle for RBCs, WBCs and platelet counting is essentially the same except that it differs in ___, ___ used, and the ___ of counting

Answer 10

dilution, diluents, area

Question 11

___ method that uses a pipet for dilution

Answer 11

pipet

Question 12

___ method that utilizes a test tube

Answer 12

test tube

Question 13

Microscopic hemocytometry is divided into three basic steps: ____. ____. ____

Answer 13

Manual dilution, Sampling to a hemocytometer or Charging, Cell counting

Question 14

____ are necessary to disperse the blood cells and to facilitate counting

Answer 14

Diluents

Question 15

What will happen if the diluent is absent upon viewing under the microscope?

Answer 15

Blood cells will overlap which makes counting difficult and cause erroneous result

Question 16

Tonicity of diluting fluid in RBC is ___ and for WBC is ___

Answer 16

isotonic, hypotonic

Question 17

Ideal diluting fluid should have __ SG to prevent ___ of cells

Answer 17

high, rapid sedimentation

Question 18

RBC diluting fluids example

Answer 18

Gower’s Fluid
Hayem’s Fluid
Toisson’s Fluid
Formol-Citrate (Dacie’s Fluid)
NSS (0.85-0.90 NaCl)
3.8% sodium Citrate
Bethel’s fluid

Question 19

Most common pipet for RBC manual counting in the Ph

Answer 19

Thoma pipet

Question 20

Dilutions ranging from __ to __ or __ to __

Answer 20

1:10, 1:1000

Question 21

distance between the slide and the special coverslip is referred as ___

Answer 21

depth

Question 22

Counting Chamber Open type:

Answer 22

Spencer, Burker, Levy, Levy-Hausser

Question 23

Counting Chamber Close type:

Answer 23

Thoma-Zeiss

Question 24

Counting Chamber - Used in standardization of vaccine

Answer 24

Petroff-Hausser

Question 25

Counting Chamber According to Ruling or Calibration

Answer 25

Thoma Turk Bass-Jones Fuchs-Rosenthal Improved Neubauer

Question 26

Most common hemocytometer used today

Answer 26

Improved Neubauer

Question 27

The platforms of IN are separated by a canal which is also known as ___

Answer 27

H-moat

Question 28

Located on each distal end of the platforms are the ___

Answer 28

V-slashes

Question 29

Distance between the coverslip and the platforms of the counting chamber is __ in depth

Answer 29

0.1 mm

Question 30

Ruled area of IN is __ by __ which is divided into __ squares

Answer 30

3mm by 3mm, 9 squares

Question 31

central large square for RBC and platelet count is divided into __ smaller squares

Answer 31

25

Question 32

area of each central smaller square is __

Answer 32

0.04mm2

Question 33

Each smaller square if further divided into __ squares

Answer 33

16

Question 34

reference range for adult males

Answer 34

4.60 - 6.00 x 10^12/L

Question 35

reference range for adult females

Answer 35

4.00 - 5.40 x 10^12/L

Question 36

___ prevents rouleaux formation and precipitation of proteins in cases of hypergammaglobulinemia and hemoglobinemia. can be used in the photometric determination of RBC count

Answer 36

Gower's fluid

Question 37

______ -possible mold growth because of insufficient preservative action - initiates rouleaux formation

Answer 37

Hayem's fluid

Question 38

___ is the stacking of RBC like the 'stack of coins'

Answer 38

Rouleaux formation

Question 39

___ - does not prevent mold formation - with stain which easily identifies nuclei (stains blue)

Answer 39

Toisson's fluid

Question 40

__ - best RBC diluting fluid - with preservative action so no mold growth - cell morphology altered

Answer 40

Formol-citrate (Dacie's Fluid)

Question 41

__ - used when no other RBC diluting fluid is available - ideal when there is excessive rouleaux formation and autoagglutination

Answer 41

NSS (0.85 - 0.90%)

Question 42

Dilution Factor formula

Answer 42

DF = total Volume of solution in the bulb / volume of blood aspirated

Question 43

Of cells per cubic mm

Answer 43

average cells counted x dilution factor / number of squares x area of one square x depth (0.1 mm)

Question 44

___ unit is the conventional unit

Answer 44

mm3

Question 45

in converting Conventional unit to SI unit, multiply the result to ___

Answer 45

10^6

Question 46

Conditions that are associated with increased RBC count

Answer 46

Polycythemia vera - abnormal proliferation of RBC tissue hypoxia dehydration low oxygen tension

Question 47

Conditions that are associated with decreased RBC count

Answer 47

anemia hemorrhage oligocythemia

Question 48

factors that affect RBC count when a patient lies down during blood collection, there is a decrease of an average of __% to the cell volume because of hemodilution

Answer 48

8%

Question 49

Plasma volume ___ during exercise

Answer 49

decreases

Question 50

factors that affect RBC count infants have ___ RBC count than adults

Answer 50

higher

Question 51

factors that affect RBC count women have ____ RBC count than men because they produce ___ that has an inhibitory action to erythropoiesis

Answer 51

lower, estrogen

Question 52

factors that affect RBC count men have ___ RBC count than women because they produce more ___ which has stimulatory effect on erythropoiesis

Answer 52

higher, androgen

Question 53

factors that affect RBC count red cell mass and hemoglobin have higher count in ___ than in ___

Answer 53

morning, evening

Question 54

_____ is based on the detection and measurement of changes in electrical resistance produced by the cell as they traverse a small aperture.

Answer 54

electronic impedance

Question 55

the number of pulses is ___ to the number of cells counted

Answer 55

proportional