HISTO LAB 1 LESSON 1

Question 1

Comes from the Greek word “_____” and
“_____” which means “small” and “to
look”

Answer 1

mikros and skopein

Question 2

Used to see objects that are too small for the

naked eye

Answer 2

microscope

Question 3

Shape, size, position, connections, colors,
number, texture, and even chemical
composition.

Answer 3

microscope

Question 4

is to get a magnified image,
in which structures may be resolved which could
not be resolved with the help of an unaided eye.

Answer 4

Microscopy

Question 5

The ratio of the size of an object
seen under a microscope to the
actual size observed with unaided
eye.
o The object appears larger under the
microscope

Answer 5

MAGNIFICATION

Question 6

Ability to distinguish two objects

from each other.

Answer 6

RESOLUTION

Question 7

The distance through which you can
move the specimen and still have it
in focus.

Answer 7

DEPTH OF FIELD

Question 8

Carries the optical part of the

microscope.

Answer 8

HEAD

Question 9

o Connects the head and the base
o Part where we hold/carry the
microscope from one place to
another

Answer 9

ARM

Question 10

o Support of the microscope
o Carry the light source of the
microscope

Answer 10

BASE

Question 10

o Support of the microscope
o Carry the light source of the
microscope

Answer 10

BASE

Question 11

a. Part where we look through the
microscope
b. Found on top
c. Standard magnification power of
each eyepiece is 10x.

Answer 11

Ocular Lens

Question 12

a. Can be move to adjust and have a
clearer magnification view of the
specimen.

Answer 12

Diopter Adjustment

Question 13

4 types of objective lens

Answer 13

a. Scanner
b. LPO
c. HPO
d. Oil Immersion

Question 14

3 major parts of microscope

Answer 14

head, arm, and base

Question 15

a. Hold the objective lens

b. Can be revolve

Answer 15

Nose piece

Question 16

a. Course, bigger movement; move the
microscope up and down
b. Fine, smaller movement;

Answer 16

Adjustment knobs

Question 17

a. Specimen is place here for viewing

Answer 17

Stage

Question 18

a. Holds the specimen in place

Answer 18

Stage clip

Question 19

a. Used to move the stage the up and

down, left and right.

Answer 19

Stage controls

Question 20

a. A hole on the microscope stage
through which the transmitted light
from the illuminator will reach on
the stage.

Answer 20

Aperture

Question 21

a. Located at the base
b. Captures the light from the external
source or the voltage (electric
microscope) to have a focus life unto
the specimen.

Answer 21

. Light source

Question 22

a. Found under the stage, next to the
diaphragm
b. Ensure clear sharp images
c. Without it, image will not be as
sharp as what we going to observe
under the microscope.

Answer 22

Condenser

Question 23

a. Found under the stage as well
b. Control the amount of light from the
light source that will pass through to
the specimen.

Answer 23

. Diaphragm

Question 24

→ Always carry your microscope with two hands, one grasping the arm or back slot and the other supporting the base. → Left- hold the base → Right- hold the arm of the microscope

Answer 24

CARRYING:

Question 25

→ Turn off light & center mechanical stage → Position the nosepiece so that the lowest scanning (4x) objective is in place. → Remove the slide from the stage and put it in the proper place. → Clean the stage and lenses with gauze and lens cleaner and wipe off any oil. → Wrap the cord around the arm. → Carefully carry with two hands and gently place the microscope in the proper cabinet.

Answer 25

PUTTING AWAY:

Question 26

→ Set the microscope on a flat, solid support and in a position where it will not easily be knocked off. → Properly coil the cord to avoid tripping over it.

Answer 26

TABLE PLACEMENT:

Question 26

→ Lenses must be clean for resolution. → Use only lens paper or gauze and cleaning solution. → Never use your finger, handkerchief, paper towels or spit to clean the lenses. → Do not remove any parts for cleaning; it only allows dust to enter the microscope.

Answer 26

CLEANING

Question 27

____ are the building blocks of all living things.

Answer 27

cells

Question 28

such as those of plants and | animals, are structurally complex.

Answer 28

Eukaryotic cells

Question 29

``` o Have nucleus and separated from the cytoplasm of the nuclear envelope o Cytoplasm contains membrane organelles: ribosomes, cytoskeleton o Have cell wall on the surface and have undergone the process of differentiation and specialization. o Compose of tissues and organize to organ-to-organ system. ```

Answer 29

Eukaryotic cells

Question 30

such as those bacteria, have | a simple organization.

Answer 30

Prokaryotic cells

Question 31

``` o Single cellular DNA which is not separated form the cytoplasm by membrane o Do not have any membrane organelles o Small ribosomes are present o Have cell wall on the surface o Reproduce thorough asexual reproduction or by amitosis or binary fission which occurs immediately after DNA replication. ```

Answer 31

Prokaryotic cells

Question 32

, as if the family tree of life on Earth | split into three main branches, called domains

Answer 32

eukarya bacteria Archaea

Question 33

: Plants, animals, fungi, and plants

Answer 33

eukarya

Question 34

Familiar, single-celled microorganisms, some of which are useful to humans and some of which cause human disease.

Answer 34

BACTERIA

Question 35

Single-celled microorganisms found in all types of environments but first discovered in extreme environments, such as hot spring.

Answer 35

archaea

Question 36

Locate the Acinar cells | and the islets of Langerhans

Answer 36

pancreas

Question 37

hold the base

Answer 37

Left

Question 38

hold the arm of the microscope

Answer 38

Right

Question 39

contains membrane organelles: ribosome, cytoskeleton

Answer 39

Cytoplasm

Question 40

The microscope was invented by _________

Answer 40

Anton Van Leeuwenhoek

Question 41

Microscope is an important tool for ____

Answer 41

Medtech

Question 42

Microscopes part and functions has been perfected over the past ____ years since it is invented

Answer 42

300 years