HISTOPATH LEC - LESSON 1 (APPARATUS) Flashcards

Question

Celloidin-embedded sections

Answer 1

Sliding microtome

Answer 2

Hand microtome

Answer 3

Laser microtome

Answer 4

Computerized microtome

Answer 5

1. Read labels before working 2. Wear laboratory coat/gown during laboratory experiments 3. Wear gloves when handling toxic and hazardous materials. 4. Never eat or drink inside laboratory 5. Never use equipment unless you are trained to use such. 6. Keep working areas clear from unnecessary materials. 7. Clean up work before leaving the laboratory 8. All infectious materials should be properly disposed in a yellow plastic bag and submitted to laboratory technician for proper disposal 9. Follow universal precautions, that is, to treat all human blood and human bodily fluids as if they were known to be infectious from HIV, HBV, and other bloodborne pathogens. 10. Never work alone in the laboratory. 11. Be familiar with the use of fire extinguisher. 12. Never do unauthorized experiments. 13. Never mouth pipette chemicals

Answer 6

1. Treat all chemicals as if it is hazardous. 2. Make sure all chemicals are clearly and properly labeled with a substance name, concentration, manufacturing and expiration date, and safety level. 3. Never store noxious chemicals inside the locker. 4. Use volatile and flammable compounds only in a fume hood. 5. Never allow a solvent to come in contact with your skin. Always use gloves. 6. Never smell a chemical. 7. Dispose of waste and broken glasses in a right container. 8. Clean up spills immediately. 9. Do not mix reagents.

Answer 7

Vibrating microtome

Answer 8

Saw microtome

Answer 9

Sliding microtome

Answer 10

2-5 degrees celcius higher than the melting point of paraffin wax

Answer 11

5 to 10 degrees celcius below than the melting point of paraffin wax

Answer 12

it serves as a container to hold the chemicals

Answer 13

it is design to store paraffin wax in solid state into liquid state

Answer 14

-10 degress celcius to -40 degress celcius

Answer 15

- microtome is a mechanical instrument used to cut biological specimens into very thin sections or segments for microscopic examinations. - it is also used for sectioning and ribboning of tissues.

Answer 16

Block holder/chuck/tissue holder Knife carrier and knife Base (Microtome body)

Answer 17

if malfunction, the tissues that have been processed will be affected.

Answer 18

laser microtome uses laser technology in which Medical technologists will not be touching anything on the sample but the machine itself will do its purpose.

Answer 19

It cannot produce very thin sections

Answer 20

Slow in action

Answer 21

``` Microtome Automatic Knife Sharpener Cryostat Automatic Tissue Processor Tissue Embedding Center Paraffin Oven Floatation water bath Coplin Jar ```

Answer 22

automatic | manual

Answer 23

Laser | Computerized

Answer 24

``` Rocking Rotary (Minot) Sledge Sliding Freezing Vibrating Ultrathin Saw Hand ```