HISTOPATHOLOGY

Question 1

Enzymes used for antigen retrieval in IHC
1. Phosphatase (AP – FOR LABELLING
ANTIBODY)
2. Trypsin
3. Lipase
4. Protease

Answer 1

2 & 4 AND PEPSIN

Question 2

Which of the following statements is incorrect?

Answer 2

NONE OF THESE

Question 3

All of the following are methods of antigen retrieval except:

Answer 3

INCINERATOR HEATING

Question 4

Advantages of enzymatic IHC except:

Answer 4

NONE OF
THESE

Question 5

Sources of polyclonal antibodies
1. Sheep
2. Goat
3. Rabbit
4. Mice

Answer 5

MICE

Question 6

Compatible counterstains for DAB
1. Brilliant green
2. Methyl violet
3. Eosin
4. Safranin

Answer 6

NONE OF THESE

Question 7

If alkaline phosphate was used to label antibodies in IHC which of the following can be used?
1. DAB
2. Fast Red TR salt
3. Amino Ethyl Carbazole
4. BCIP

Answer 7

2 & 4

Question 8

Which of the following chromogens that when used will produce a bluish violet or black violet color?

Answer 8

BCIP

Question 9

In IHC, what is the incubation time and
temperature to link antibody with enzyme?

Answer 9

30-60 MINUTES AT RT

Question 10

Which of the following is the most commonly used enzyme label in IHC?

Answer 10

HRP

Question 11

In IHC, which form of hematoxylin that when used tissue morphology are well defined?

Answer 11

HARRIS

Question 12

Acceptable specimens for IHC except:

Answer 12

NONE OF
THESE

Question 13

Which of the following is an oncofetal antigen that is very useful differentiating mesothelioma and adenocarcinoma.

Answer 13

CEA

Question 14

Which is highly specific for myogenic tumor?

Answer 14

DESMIN

Question 15

Which of the following is the marker for
choriocarcinoma?

Answer 15

HCG

Question 16

The tumor marker that will confirm diagnosis of
astrocytoma?

Answer 16

GFAP

Question 17

An epithelial tumor marker which is also positive in certain pancreatic and salivary gland cancer

Answer 17

PSA

Question 18

Conditions that will stan positive for vimentin.

Answer 18

ALL OF THESE

Question 19

Which of the following is synthesized by placental syncitioltrophoblast?

Answer 19

HCG

Question 20

All are intermediate filament markers except:

Answer 20

TTF-1

Question 21

Which of the following is more common in
carcinomas of the colon and stomach?

Answer 21

CK 20

Question 22

Which can be helpful in determining tumor site?

Answer 22

EMA

Question 23

Most common form of antibody used in IHC?

Answer 23

IgG

Question 24

True of IHC except:

Answer 24

D. SPECIMEN FOR IHC IS
LIMITED ONLY TO PRICESSED SPECIMEN LIKE
PARAFFIN EMBEDDED TISSUES

Question 25

Uses of this chromogen can lead to production of brick red color.

Answer 25

AEC

Question 26

Cells found in cervico vaginal smear with a diameter of 15-30u

Answer 26

PARABASAL CELLS

Question 27

Which cell has basophilic vacuolated cytoplasm.

Answer 27

INTERMEDIATE CELLS

Question 28

In Pap’s staining of non-gynecological specimens. Which form of hematoxylin is used as nuclear stain?

Answer 28

HARRIS

Question 29

Which of the following are used as counterstains in Pap’s staining?

Answer 29

2 & 4

Question 30

Which of the following cells in cervico vaginal smear is shed in response to ovarian hormone?

Answer 30

ENDOMETRIAL CELLS

Question 31

These are boat shaped cells with a tendency to fold or curl on edges.

Answer 31

NAVICULAR CELLS

Question 32

Which may assume a “honeycomb” appearance?

Answer 32

ENDOCERVICAL CELLS

Question 33

The presence of these cells suggests a combine estrogen-progesterone effect?

Answer 33

NAVICULAR CELLS

Question 34

Predominance of which of the following cells is noted following childbirth?

Answer 34

PARABASAL CELLS

Question 35

These are polygonal squamous cells measuring 45-50um in diameter.

Answer 35

SUPERFICIAL CELLS

Question 36

In Pap’s staining of gynecological specimens. Which form of hematoxylin is used as nuclear stain?

Answer 36

HARRIS

Question 37

In Pap’s staining of the following will that when used will stain the cytoplasm of other cells including non-keratinized squamous cells.

Answer 37

LIGHT GREEN SF

Question 38

In cytochemistry, which can be used to stain glycogen?

Answer 38

PERIODIC ACID SCHIFF

Question 39

Which is considered to be that staining method of choice in exfoliative cytology.

Answer 39

PAPANICOLAOU

Question 40

All of the following statement are correct except:

Answer 40

E. NONE OF THESE