Infiltration / Impregnation and Embedding (F) Flashcards
(111 cards)
What is the process (/ steps) in conventional tissue processing (include all minor steps)?
1) Labeling (Numbering)
2) Fixation
2. 1) Washing out
3) Decalcification (optional)
4) Dehydration
5) Clearing
6) Impregnation (Infiltration)
6. 1) Orientation
7) Embedding
7. 1) Trimming
8) Section-Cutting (Microtomy)
8. 1) Floating Out
8. 2) Adhesion
9) Staining
10) Mounting
10. 1) Ringing
11) Labeling
True or False
Tissue processing starts w/ labeling and ends w/ mounting
False, because tissue processing starts w/ labeling and ends w/ labeling
What is the workflow (/ steps) in the histopath lab)?
1) Grossing
2) Freezing
3) Fixation
4) Decalcification (optional)
5) Processing (/ automated tissue processing)
6) Embedding
7) Sectioning
8) Staining (special / routine [H & E staining])
8. 1) LMD
9) Coverslipping
10) Reading
11) Digitalizing
12) Storage
What is infiltration / impregnation?
It is the process of removal of clearing agent
What is the principle (/ action) done in infiltration?
Fill the tissue cavities
Why are the tissue cavities filled in infiltration?
To give a firm consistency
What are the results of infiltration?
1) It promotes easier handling
2) It promotes ease of cutting into thin sections
After infiltration, why is ease of cutting into thin sections achieved?
Due to the firmer consistency present
What are the results if inadequate impregnation is present?
Tissue becomes:
1) Soft
2) Crumbly
Why is infiltration done?
Because tissue blocks crumble when sectioned and break up when floated out in a H2O bath
What are the other terms for embedding?
1) Blocking
2) Casting
What is embedding (/ what is its principle)?
It is the process by w/c the impregnated tissue is placed into a precisely arranged position in a mold containing a medium w/c is then allowed to solidify
What are the 3 concepts associated w/ embedding?
1) Orientation
2) Markers
3) Troubleshooting
What is orientation?
It is how the MT places the tissue (w/c is presented to the patho)
What is the purpose of markers?
These are placed to know the base of the tissues
True or False
The medium used to infiltrate the tissue is usually the same medium utilized for impregnation
True
The medium used to infiltrate the tissue w/c is usually the same medium utilized for impregnation in terms of general purpose is known as what?
Embedding medium / resin
What are the usually used impregnation and embedding media?
1) Paraffin wax
2) Celloidin
3) Gelatin
4) Plastic
What should be the characteristics of an infiltrating and embedding medium?
1) It should be soluble in processing fluids
2) It should be suitable for sectioning and ribboning
3) It is molten between 30 DC and 60 DC
4) It should be translucent or transparent
5) It should be colorless
6) It should be stable
7) It should be homogenous
8) It should be capable of flattening after ribboning
9) It should be non-toxic
10) It should be odorless
11) It should be easy to handle
12) It should be non-expensive
What are the factors affecting infiltration / embedding?
1) Laboratory temperature
2) Number of changes
3) Clearing agent used
4) Volume
What should be the lab temp?
Room temp (20 - 24 DC)
What should be the temp of the paraffin oven?
2 - 5 DC higher than the melting point of the wax
What are the results if the lab temp is beyond 60 DC?
It is deleterious to the tissue; it causes the ff to the tissue:
1) Shrinkage
2) Dryness
3) Brittleness
4) Difficult to section
How many number of changes should be done?
At least 2 changes