Introduction - Histology (L1)

Question 1

Staining

Answer 1

Most common is “H&E”: hematoxylin and eosin together.

The basic dye hematoxylin stains acidic structures a purplish blue; those structures are basophilic (eg nuclei, rough ER).

The acidic dye eosin stains basic structures red or pink; those structures are acidophilic (eg cytoplasmic proteins, and hence cytoplasm).

In animal cells: nuclei stain BLUE and cytoplasm stains PINK or RED.

Question 2

Epithelium outline in classification of animal tissues

Answer 2

I. Epithelium
A. Simple epithelium (squamous, cuboidal, or columnar)
B. Stratified epithelium (stratified squamous, stratified cuboidal)
C. Others (Transition, Pseudostratified ciliated columnar)

Question 3

Connective Tissue outline in classification of animal tissues

Answer 3

II. Connective Tissue
A. Vascular tissue (blood, lymph)
B. Connective tissue proper (loose connective tissue, dense connective tissue)
C. Cartilage
D. Bone

Question 4

Muscle/Nerve outline in classification of animal tissues

Answer 4

III. Muscle
A. Skeletal
B. Smooth
C. Cardiac

IV. Nerve