Flashcards in L12 - Autism mouse models Deck (15):
What are 3 facets of Autism spectrum disorder?
reprtitve/restricted behaviours - serious enough to effect everyday life
What are the co-mordbidities of ASD?
motor disorders - distinctive gait
sensory deficits (90%)
ASD affects about __% of school aged children
True or false
ASD is Heterogenous and a lifelong disorder
Which functional gene groups are implicated in ASD?
Sex hormones - e.g estrogen receptor
Neural development and connectivity (Neuroligin-3)
immune pathways - IL10
Why do we use mice in brain research?
They have a six layered neocortex
short breeding and lifespans
mice are inbred and geneticially identical, can assess effect of environmental factors
can look at the molecular basis of neurological disease with invasive techniques
What are the Advantages of mouse models?
Genome accessible for manipulation
Difficult to model entire spectrum of disease phenotypes but can focus on specific traits or ''endophenotypes'' of the disorders
- repetitive actions
- social behaviour
What makes a good mouse model?
- Endophenotypes: eg: Behavioural traits similar to patients
- Biological dysfunction: eg Gene mutations found in patients
- response to treatments: eg atypical antipscychotics
How to study behaviour in mouse models of autism?
associated symptoms: sezures, anxiety, sleep disruption etc..
How do you measure social interaction in mouse?
3 chamber test
agression = resident intruder test
How do you measure brain mechanisms in mouse autims models?
Electrophysiology - EEG
molcular biology - single cell RT-PCR
Anatomy - biocytin labelling, immunochemistry
What is the Neuroligin-3 mouse (NL3) model of autism?
Neuroligin-3 is a postsynaptic adhesion moelcule which mediates synaptic signalling via binding to neurexins
This is reduced in the model, - same amount of mRNA but in the processing the concenrtation at the synapse is reduced
has incerased aggression phenotype
What are the anxiety tests for mouse?
elevated plus maze
true or false
in the resident intruder test, mutatnt NL3 muce are quicker to attack