L12 - Autism mouse models Flashcards Preview

Seminars in Translational Medicine > L12 - Autism mouse models > Flashcards

Flashcards in L12 - Autism mouse models Deck (15):
1

What are 3 facets of Autism spectrum disorder?

Social interaction

communication

reprtitve/restricted behaviours - serious enough to effect everyday life

2

What are the co-mordbidities of ASD?

GIT symptoms

sleep disorders


seizures

aggression

axiety

motor disorders - distinctive gait

sensory deficits (90%)

3

ASD affects about __% of school aged children

1.5%

4

True or false

ASD is Heterogenous and a lifelong disorder

true

5

Which functional gene groups are implicated in ASD?

Sex hormones - e.g estrogen receptor

social responsivity

Neural development and connectivity (Neuroligin-3)

immune pathways - IL10

Metabolism

6

Why do we use mice in brain research?

They have a six layered neocortex

short breeding and lifespans

mice are inbred and geneticially identical, can assess effect of environmental factors

can look at the molecular basis of neurological disease with invasive techniques

7

What are the Advantages of mouse models?

Genome accessible for manipulation

Difficult to model entire spectrum of disease phenotypes but can focus on specific traits or ''endophenotypes'' of the disorders
- communication
- repetitive actions
- social behaviour

8

IN EXAM

What makes a good mouse model?

face validity
- Endophenotypes: eg: Behavioural traits similar to patients

Construct validity
- Biological dysfunction: eg Gene mutations found in patients

Predictive validity
- response to treatments: eg atypical antipscychotics

9

How to study behaviour in mouse models of autism?

Social interaction

communication

repetitive behavious

associated symptoms: sezures, anxiety, sleep disruption etc..

10

How do you measure social interaction in mouse?

3 chamber test

agression = resident intruder test

11

How do you measure brain mechanisms in mouse autims models?

Electrophysiology - EEG

molcular biology - single cell RT-PCR

Anatomy - biocytin labelling, immunochemistry

fMRI

12

What is the Neuroligin-3 mouse (NL3) model of autism?

Neuroligin-3 is a postsynaptic adhesion moelcule which mediates synaptic signalling via binding to neurexins

This is reduced in the model, - same amount of mRNA but in the processing the concenrtation at the synapse is reduced

has incerased aggression phenotype

13

What are the anxiety tests for mouse?

elevated plus maze

light-dark box

14

true or false

in the resident intruder test, mutatnt NL3 muce are quicker to attack

true

15

What rescues the enhanced agression in NL3 mice?

risperidone