Flashcards in L7 inher of genes Deck (4)
LO1: desc proc and role DNA replic.
-2 str sep= templs. catal by DNAP- can ext 3', add complem base, P on new base joins OH on 3' of primer str. PPi pryoP rel.
dNMP primer +dNTP substr forms dNMPn+1 + PPi.
DNA helicase seps str. stepw 5' to 3' chain growth. reac driven by PPi hydrol. semi cons.
-init- recog seq ori of replic. req recruit DNAP and other specif prots. reqs kick start by primase as DNAP can only ext 3' end. primase makes few nt RNA first, DNAP elongs, get rid.
-elong- ext 3'. DNAP read templ 3' to 5'= lead str synth contin. on other str DNAP binds at fork, replic ag direc of replic as strs open, discontin okazaki frags made on lagg str. DNA ligase joins. moving replic forks.
-termin- 2 forks come togeth. ligase. each old str has complem new.
-one chrom=1 dsDNA molec. one chrom also= 2DNA molecs-one replic chrom, so chrom numb const. sis chromatids ident. non sis on mat and pat homol pair non identic. metacentric, submetacentric, acrocentric, telocentric.
LO2: desc proc and role of cell cyc. complex contr.
-somat cell diploid 2 chrom pairs. gamete hap 23 chroms.
-G0 outs cyc, not div anymore eg nerve, can re-ent.
-gap 1 cell content replic.
cell cyc checkpt, can go to apop.
-S- DNA replic. synthesis. 8 hr.
-G2 doub check and rep, main DNA.
cell cyc checkpt.
-M- cell div. x chrom as replic already. sis chromatids.
LO3: desc proc and role mitosis and meiosis, comp and contrast.
MITOSIS: 1 rnd replic, 1 div.
-cell div for somat cells. dip cell to 2 dip daught ident. mitot growth req some tiss eg epidermis, mucosae, BM, spermatogonia. growth/maint. not nerve cell.
PROPH- nuc mem disint. pair centrioles and spind fibs app, chrom condense.
PROMETAPH- centrioles to poles. spind fibs connec kinetochore prot complex which specif bound centromere. chroms cond.
METAPH- chroms line up metaph plate. rand ord incl homol pairs.
ANAPH- centrom div. chromatids split pulled tow opp poles by kinetoch fibs. =daught chrom.
TELOPH- nuc mem reform. chrom decond. spind disapp. cleavage furrow forms.