Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

BIOL1115 LAB > Lab 1 > Flashcards

Lab 1 Flashcards

(54 cards)

Start Studying
1
Q

Ocular Lenses

A

located inside eyepiece, magnifies specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Objective lenses

A

Series of lenses located on nosepiece, magnifies specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Arm

A

Used for hand grip when carrying

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Coarse Focus Knob

A

Roughly focuses specimen by changing distance between objective lens and specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Light Source

A

Emits light onto specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Blue Filter

A

Increases Resolution by reducing light wavelength

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Mechanical Stage

A

large, flat surface that supports slide/specimen over the hole that admits light from below, allows for movement of the slide using mechanical stage knobs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Mechanical Stage Knobs

A

moves slide around stage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Fine Focus Knob

A

Sharply focuses specimen by changing distance between objective lens and specimen very precisely

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Condenser

A

Contains 2 lenses that focus light on specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Iris Diaphragm

A

regulates amt of light passing through lenses in the condenser, increases visibility of semi-transparent objects

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Iris Diaphragm Lever

A

opens and closes iris diaphragm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Condenser Adjustment Knob

A

Moves Condenser up and down

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Lamp Switch

A

turns light on or off

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

lamp intensity dial

A

adjusts brightness produced by light source

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

stage

A

large flat surface supporting slide over hole that admits light from below

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

main switch

A

controls supply of power to microscope, must be on for either light to work

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

T switch

A

turns light BELOW specimen on or off

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

I switch

A

turns light ABOVE specimen on or off

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Stage Clips

A

holds a slide in position over stage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

unaided eye resolution is

A

0.1mm in length

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

max magnification of compound light microscopes

23
Q

max resolution of compound light microscopes

Study These Flashcards
A

0.2micrometers, 0.4micrometers without immersion oil

24
Q

what are properties of image produced by compound light microscopes?

Study These Flashcards
A

images are inverted and reversed

25
what are properties of image produced by dissecting light microscopes?
images are right side up
26
magnification
enlargement of an image
27
depth of field
vertical distance that remains in focus at each magnification
27
resolution
clarity and sharpness of an image, minimum distance that 2 points can be separate and still distinguished as 2 units
28
what is resolution affected by?
wavelength of light because the longer the wavelength, the more the diffraction, resulting in blurry image whereas the shorter the wavelength, the less the diffraction resulting in sharper image
29
how do you improve resolution?
- using blue filter to reduce wavelength of light that enters condenser - using immersion oil that decreases diffraction, increasing chances of light waves entering objective lens
30
contrast
quality that makes image stand out better, the ability to discern detail
31
how do you improve contrast?
- stain layers between tissues and cells of thin sectioned specimen using artificial dyes - use speical light microscopes that use diff types of light to make image clearer or easier to see - reduce amount of light using either iris diaphragm or light intensity dial or adjusting condenser
32
What is the relationship between magnification and depth of field?
the higher the magnification, the smaller the depth of field
33
Why must a microscope increase magnification AND resolution?
high magnification is NOT valuable without good resolution
34
How do electron microscopes produce images?
Uses electron beams with very short wavelengths
35
What is resolution of Electron Microscopes?
0.2nanometers or 0.0002micrometers
36
What is process of using Transmission Electron Microscopes?
Specimen undergoes dehydration process, embedded in resin that solidifies Sliced thinly with a microtome Slices are put onto small copper grid and stained before being placed into the TEM in a vacuum Specimen and grid are mounted inside objective lens Electron beam passes through specimen, and electromagnets are used as lenses to focus and magnify image As electrons pass through sample, they are deflected from stained portions and transmitted through unstained areas Electron microscopes can view specimens 50-100µm thick
37
What is process of using Scanning Electron Microscopes?
Specimen is put on small copper grid and coated with thin layer of gold/platinum and put in the SEM in a vacuum A beam of electrons bombard specimen surface and scams back and forth across it, produces image w/ 3D detail and great external detail Electrons do NOT pass through specimen Depth of field greater than that if any other microscope
38
How do you calculate actual size of specimen?
Actual Size(mm) = fraction of field occupied x field diameter(mm)
39
How do you calculate speed of specimen?
Speed of specimen (mm/s) = distance 9mm) ÷ time (s)
40
How do you calculate magnification?
Magnification = size of image (mm) ÷ actual size (mm)
41
How do you convert between milimeters, micrometers, and nanometers?
1cm = 10mm 1mm = 1000µm 1µm = 1000nm
42
parfocal
an object in focus at low power should be relatively focused when you switch to higher powers, small adjustment on fine focus is all that is necessary
43
Why do you not move stage down before switching objective lenses?
you will go out of focus
44
Why do you pick up coverslips by the edges?
so you won't smudge it or get fingerprints on it
45
field diameter of low power objective lens
4.5mm
46
field diameter of medium power objective lens
1.8mm
47
field diameter of high power objective lens
0.4mm
48
Which kind of microscope can you view live specimens?
light microscopes, electron microscope specimens are dead
49
Which microscope produces 3D images?
Dissecting Light microscope and Scanning electron microscope, transmission electron microscope and compound light microscope produces 2D images
50
Which kind of microscopes can you use to see external structures and entire specimens?
Scanning Electron Microscopes and Dissecting Microscopes, Transmission Electron and Compound Microscopes are used to view internal, sectioned specimens
51
What is maximum magnification of electron microscopes?
300,000x
52
When should you never use the coarse focus knob when using compound light microscopes?
When using the medium and high power objective lenses, you might smash lenses into the slide and because you might lose focus of the specimen by roughly focusing specimen
53

BIOL1115 LAB (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions