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Flashcards in LAB 1 Deck (32)
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1

COLONY

small mass of genetically identical cells

2

PURE CULTURE

isolated group of genetically identical cells

3

MEDIUM
- LAWN

solution or solid with nutrients so that cells can grow/ divide.
It can be a liquid or a mixed with something to solidify it.
ex: Agar plate= solid medium that helps cells grow and divide.
- dense, even layer of bacteria.

4

LIGHT MICROSCOPE (includes 3 lenses)

Light from light source shines on specimen and lenses magnify the image.

5

CONDENSER LENS

Focuses light from light source to specimen.

6

OBJECTIVE LENS

Magnifies image on specimen

7

OCULAR LENS/EYPIECE

Magnifies image+ INVERTS it for better viewing.CONTAINS MICROMETER

8

FIELD IRIS DIAPHRAGM

Iris that controls the light that enters the lens system. Decreasing Light improves contrast.

9

CONTRAST

Difference between light and dark areas. Can make translucent images stand out.

10

COURSE FOCUS KNOB

Moves Stage up and down. Only used for LOW power lenses.

11

FINE FOCUS KNOB

Bring image to SHARPER focus under Low power. HIGH power lenses.

12

BODY TUBE

Tube that contains a prism that allows light to pass from objective lens to ocular lens.

13

INTEPUPPINARY DISTANCE

Distance between your pupils. Incorrectly set can cause eye strain, incorrect measurements, and may affect microscope’s ability to be par-focal

14

TOTAL MAGNIFICATION

Objective lens X Ocular lens
eg: 40/0.2= Objective magnifies image 40 times its actual size.

15

PAR FOCAL

objective lens can be changed without having to refocus microscope

16

RESOLUTION

Differentiates two adjacent points as being different.

17

RESOLUTION VALUE

minimum distance required for two objects to be seen as separate.

18

Hypothesis

educated guess that predicts the answer to your question

19

Constants

factors which are kept constant throughout the experiment

20

INDEPENDENT VARIABLE

variable of that is MANIPULATED

21

DEPENDENT VARIABLE

RESULT OF MANIPULATION

22

EXPERIMENTAL GROUP

measures the effect of Independent variable(manipulated variable) on the dependent variable.

23

CONTROL GROUP

examines dependent variable in the absence of the independent variable.

24

SOLVENT CONTROL GROUP

examines solvent in experiment. Makes sure that the effect on the dependent variable is only due to the independent variable and not due to the solvent.

25

OSMOSIS

water movement from area of [low solute] to [high solute]
-Passive transport
Plants, prokaryotes, protists have cell walls that constrict the cytoplasm size and prevent plasma membrane from rupturing.

26

HYPOTONIC

SOLUTION with [LOW SOLUTE] than cell. TURGID: cell swells due to water entering the cell.

27

HYPERTONIC

SOLUTION with [HIGH SOLUTE] than cell. Plasmolysis: cell shrinks as water leaves the cell, Plasma membrane disrupted and cell dies.

28

ISOTONIC

SOLUTION with same [SOLUTE] as cell. FLACCID: no net mvmt of water, low pressure on cell wall.

29

CALACUALTIONS

1MM=10000UM
1UM=1000NM

30

STAGE

surface where slide is placed. Horizontal mvmt: object can be located. Vertical mvmt: focus on slide.