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Flashcards in LAB 3 Deck (20)
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1

MICROBIOTA

bacteria growing on and in our bodies.
bacteria can be pathogenic, mutualistic, commensal

2

What is the best way to isolate individual cells?

Streaking them onto an agar plate so that an individual cell can form a colony.

3

ASEPTIC/STERILE TECHNIQUE
1) STERILE
2)REDUCE EXPOSURE TIME
3)WORK IN A LOW RESISDENT POPULATION AREA OF BACTERIA.

prevent environmental bacteria to contaminate work. REDUCES CONTAMINATION.
1) wash hands before and after handling microorganisms
-Flame items using Bunsen burner
- Glassware+ medium is sterilized using high temp and pressure using autoclave.

4

ZONE OF INHIBIOTION

area where antibiotic sensitive bact. are unable to grow.

5

NO ZONE OF INHIBITION

Antibiotic resistant bacteria

6

ANTIBIOTICS

Prevent bacterial and fungal growth.

7

16s ribosome sequence

rRNA sequencing or entire genome sequencing to determine diversity of microbiota.

8

ANITBIOTICS FUNTIONS

1) block cell wall formation or damage it's integrity
2) block protein synthesis
3)stop DNA replication or transcription.

9

ANTIBIOTIC RESISTANCE

bacterial ability to actively resist antibiotic function.

10

TETRACYLINE

acts against UTI, STI to plague.
prevents bacterial protein synthesis.

11

PENICILLIN

treats Gram-positive bacteria
-prevents crosslinking of bacterial cell wall

12

CHLORAMPHENICOL

only used for pink eye in North America due to widespread antibiotic resistance.
-more widely used in developing countries.
-inhibits bacterial protein synthesis

13

How is Antibiotic Resistance spread?
1) Mutation of bacterium's genes
2) Horizontal transfer

1) one or two small changes in organism that makes it antibiotic resistant.
2) one organism passes genes for antibiotic resistance to the other usually in the form of plasmids(small, circular DNA)

14

How is antibiotic resistance achieved?

1) modify the antibiotic via degradation or alteration.
2) block influx and cause efflux of antibiotic.
3) "global adaption"- alteration in a major component in cells that prevents antibiotic action.
-Makes cells less viable, overall, in the absence of antibiotic.

15

GROWTH PROMOTERS

used as additives
-preventative medicine to reduce disease in high density feedlots (livestock)

16

FIRE BLIGHT

-condition on apple and pear trees.
-tetracycline and streptomycin is used to treat this condition.

17

GRAM POSITIVE BACTERIA

- one memb
-thick layer of peptidoglycan in cell wall
-stains purple

18

GRAM NEGATIVE BACTERIA

thin layer of pept. btw 2 memb's.
stains pink.

19

MICROBIOME

collection of organisms found in our body.

20

VIRULENCE

potential harm bacteria causes.