Lab 13

Question 1

Lab goal - 13

Answer 1

Measure rate of fermentation by CO2 production in yeast run descriptive stats and variability

Question 2

Glucose Reaction

Answer 2

C6H12O6 -> 2C2H3OH + 2CO2

Question 3

Repeat vs replicate

Answer 3

Repeat done in same experimental run, replicate identical but different runs

Question 4

How to convert from volume to mols?

Answer 4

PV=nRT find n

Question 5

How were rates found and compared

Answer 5

Fermentation rate is slope from scatter plot compared across class w/ descriptive stats

Question 6

What controls were used lab 13

Answer 6

Find baseline CO2 production without yeast fermentation, plot line and subtract from experimental data

Question 7

Hypothesis vs. prediction

Answer 7

Educated guess to question vs. predicted outcome based on experimental design considering each variable.

Question 8

Overall Reaction yeast

Answer 8

C6H12O6+2ADP+2P=2 ethanol+2CO2+2ATP

Question 9

Glycolysis and alcohol fermentation

Answer 9

Glycolysis splits glucose and adds electrons to 2 NAD+ (makes NADH&2H+) using 2 ADP + 2 P. Creates 2 pyruvate; which split into 2 acetaldehyde and 2CO2. 2 Acetaldehyde is converted to 2 Ethanol by alcohol fermentation - which uses the electrons carried by 2 NADH, restoring them to 2NAD+.

Question 10

What is the purpose of the baseline CO2 control?

Answer 10

Yeast cells produce CO2 outside of alcohol fermentation, and even outside of the cells. want to just isolate this process

Question 11

What were the two control cups

Answer 11

No glucose cup and no yeast cup to get total baseline control

Question 12

What independent variable is being tested lab 13

Answer 12

[yeast] NOT [glucose]

Question 13

What is the point of the NaP buffer

Answer 13

Maintains oh range for enzyme activity