Lab 24 Flashcards
(4 cards)
1
Q
BLAST is used to _____-
A
determine if there are off-target sites of gRNA
2
Q
gRNA must be
A
20 nucleotides of homologous sequence just upstream of PAM site, 40-80% G/Cs including the gRNA and 3 PAM site, and should be unique in the genome of interest
3
Q
Chopchop requires
A
target, species, enzyme/mechanism,knockout/editing/function
4
Q
A
