Lab 15 Flashcards
(6 cards)
1
Q
Focuses lab 15
A
PET data correction, developing controls, pigment chromatography and chlorophyll fluorescence
2
Q
What is Rf in paper chromatography
A
Retardation factor - distance traveled by medium out of total distance of solvent front
3
Q
What do we expect to see in chlorophyll chromatography
A
Chlorophyll a, chlorophyll b, B-carotenes (bright yellow), xanthophylls (pale yellow) - order should be carotene, xanth, a, b from furthest to least travelled
4
Q
Which ones fluoresce
A
Only chlorophylls fluoresce
5
Q
Why does chlorophyll a move further than b
A
More polar (less non polar groups on outside)
6
Q
Positive vs negative controls
A
Positive - known to create change, will produce result. Negative - known to have no effect, no change expected
