Lab 13

Question 1

refers to the information about the metabolism of bacteria

Answer 1

Biologic Characters

Question 2

the chemical reactions that occur within all living organisms

Answer 2

metabolism

Question 3

proteins that catalyze biological reactions

Answer 3

enzymes

Question 4

the majority of enzyme which function inside a cell

Answer 4

endoenzymes

Question 5

many bacteria make these enzymes, which are released from the cell to catalyze reactions outside the cell

Answer 5

exoenzymes

Question 6

chemical reactions that release energy from the decomposition of complex organic molecules

Answer 6

catabolism

Question 7

organic molecules that contain carbon, hydrogen, and oxygen in the ration (CH2O)n

Answer 7

carbohydrates

Question 8

simple sugars containing 3-7 carbons

Answer 8

monosaccharides

Question 9

composed of 2-20 monosaccharide molecules

Answer 9

oligosaccharides

Question 10

the most common oligosaccharide

Answer 10

disaccharides

Question 11

consist of 20 or more monosaccharide molecules

Answer 11

polysaccharides

Question 12

Exoenzymes are mainly _________ that leave the cell and break down, by the addition of water, large substrates into smaller components, which can then be transported into the cell.

Answer 12

hydrolytic enzymes

Question 13

hydrolyzes the polysaccharide starch into smaller carbohydrates

Answer 13

amylase

Question 14

a monosaccharide that can be released by hydrolysis

Answer 14

glucose

Question 15

chemical reaction that requires the presence of molecular oxygen

Answer 15

oxidative catabolism

Question 16

The presence of an _____ is determined by looking for a change in the substrate outside a bacterial colony.

Answer 16

exoenzyme

Question 17

chemical reaction that does not require oxygen but may occur in its presence

Answer 17

fermentative catabolism

Question 18

nutrient semisolid agar deep containing a high concentration of carbohydrate and a low concentration of peptone

Answer 18

OF medium

Question 19

In OF medium, the ____ will support the growth of bacteria that don’t use the carbohydrate.

Answer 19

peptone

Question 20

OF medium contains the indicator ______, which turns _____ in the presence of acids, indicating _____ of the carbohydrate.

Answer 20

OF medium contains the indicator bromthymol blue, which turns yellow in the presence of acids, indicating catabolism of the carbohydrate.

Question 21

________, caused by he use of the peptone and not of carbohydrate, are indicated by a dark blue color due to ________ production.

Answer 21

Alkaline conditions, caused by the used of peptone and not of carbohydrate, are indicated by a dark blue color due to ammonia production.

Question 22

If the carbohydrate is metabolized in both tubes, _____ has occurred.

Answer 22

If the carbohydrate is metabolized in both tubes, fermentation has occurred.