Lab 2

Question 1

Pure Culture

Answer 1

A single species or strain of bacteria growing in culture , cloned identical cell colonies

Question 2

Most common procedures to for isolation

Answer 2

Isolation or quadrant steak method

Question 3

Failure to flame between quadrants

Answer 3

Would not create a isolation streak because the flame is one of the ways to reduce bacteria throughout process

Question 4

Streaks not parallel or over lapping

Answer 4

you will pull too many cells into the next area and will not reduce the number enough to get isolated colonies

Question 5

Transfer from one place of living to a new place

Answer 5

Sub culture

Question 6

is a common culture medium inoculated by a straight streak of organism from bottom to top with either a loop or needle,

Answer 6

Slant medium

Question 7

Characteristics observed on slant culture

Answer 7

Amount
Color
Shape
Opacity ( transparency, translucent)
Odor

Question 8

Uniform dispersion of the bacteria within the liquid will lead to, or cloudiness.

Middle of broth

Answer 8

Turbidity

Question 9

Some organisms will settle out, or fall to the bottom of the tube.

Answer 9

Sediment

Question 10

Another character to look for in broths is the presence of a pellicle, or skim, on the surface of the broth. (If the broth is agi-tated, this will often fall.)

Top

Answer 10

Pellicle

Question 11

Isolated colonies

Answer 11

Size
Color
Margin
Shape
Elevation

Question 12

A temperature at which the enzyme functions at its highest rate

Answer 12

Optimal temperature

Question 13

The structure enzymes is altered it may no longer attach to appropicate substrate

Answer 13

Denature

Question 14

Bacteria species that will grow in a range from 5 up to 20

Organisms live in cold water soils and fridge

Answer 14

Psychrophiles

Question 15

The only human pathogen that grownphsychrophilically listeria

Answer 15

monocytogenes

Question 16

Bacterial species which grow within the range from just below 20- up to 45

Humans and other organisms live

Answer 16

Mesophiles

Question 17

Growing form 20-35 these are plants pathogens

Decomp of plants

Answer 17

Room temp mesophiles

Question 18

Bacterial species which have thermostable enzymes flourish in hot environment

Growing at 45 and higher

Answer 18

Thermophiles

Question 19

Found on warm blooded animals as mutualistic normal flora or pathogens 35-45

Answer 19

Body temp mesophiles

Question 20

Rapidly attach two molecules and change them into harmless water and 02 molecules

Answer 20

Catalase

Question 21

Flexiable group of bacteria based on oxygen

Answer 21

Facilitative

Question 22

Small air loving

Answer 22

Microaerophiles

Question 23

Blue strip
Gas pak growth of microaeropjoles in jar

Answer 23

Anaerobe system

Question 24

Anaerobic and facultative

Answer 24

Candle jar

Question 25

Anaerobic and facultative

Answer 25

Candle jar

Question 26

Chemicals used on living surfaces such as skin to kill or inhibit the growth of bacteria

Answer 26

Antiseptics

Question 27

Too harsh for living tissues and instead used to kill or inhibit microbes growth on non living surfaces

Answer 27

Disinfectant

Question 28

Chemicals used in the treatment of disease rather than just to inhibit or kill

Answer 28

Antibiotic

Question 29

A sense of growth around the disk by clear area called

Answer 29

Zone of inhibition