Lab 2 Pre-quiz Flashcards Preview

Anatomy and Physiology 1 Lab > Lab 2 Pre-quiz > Flashcards

Flashcards in Lab 2 Pre-quiz Deck (29):
1

Binocular tube

Holds 2 eyepieces or oculars

2

Oculars (eyepieces)

2 lenses positioned in binocular tube; magnifies image 10x

3

Diopter adjustment ring

ring at base of left eyepiece; adjusted according to interpupillary distance

4

Stand/arm

used to carry microscope

5

Revolving nosepiece

revolving plate which holds objective lenses

6

Objectives

4 lenses which magnify the image:
1. Scanning objective: magnifies 4x
2. Low power objective: magnifies 10x
3. high power objective: magnifies 40x
4. oil immersion objective (longest objective): magnifies 100x

7

Stage

Platform containing a central opening; contains specimen holder with a spring-loaded finger

8

Mechanical stage

Device which holds the microscope slide in place and moves it along the stage. The control knobs for the mechanical stage are located below the stage and move the slide from side to side and forwards and backwards

9

Condenser

group of lenses below the stage which directs and concentrates the light beam from the lamp to the specimen

10

Diaphragm lever

adjusts the amount of light passing through the condenser; controls brightness

11

Course adjustment knob
•how does it focus the image
•what happens went turned clockwise
•what happens when turned counter clockwise

•Focuses image by raising and lowering the stage
• raises the stage
•lowers the stage

12

Fine adjustment knob

small knob in center of course adjustment knob used for final focusing

13

Base

bottom support; houses lamp

14

Lamp
•what is it
•what kind of bulb

•Light source
•tungsten bulb

15

Voltage control dial or slide

control intensity of light from lamp

16

Power switch

switch on front which turns on lamp

17

Care of the compound microscope
•7 steps

1. Use the microscope assigned to your desk. Obtain the microscope and power cord from the cabinet
2. To carry the microscope, hold the arm with one hand and the base with the other
3. Keep your microscope clean. Use only lens paper to clean the lenses. Do not use paper towels or tissues
4. Never take the microscope apart
5. Do not let the cord hang below the table or become covered with books. It can accidentally be pulled from the table
6. Place the microscope flat on the table at least 4 in. from the edge. Clear everything off the table
7. Report any malfunctions

18

Resolution

ability to distinguish between 2 points that are close together

19

Field of view

The circle of light

20

Depth of field

thickness of a specimen that can be seen in sharp focus at the same time

21

Working distance

Space between the bottom of the objective and the specimen

22

How to prepare a wetmount
•5 steps

1. Obtain a clean microscope slide and cover slip
2. Place a drop of methylene blue in the center of the slide
3. Gently scrape the inside of your cheek with a clean toothpick
4. Mix the cheek cells with the methylene blue on the slide
5. Gently apply the cover slip

23

Compound microscope
•what does it do
•why is it called compound

•It is used to enlarge the image of the specimen
• because it requires 2 lenses for magnification

24

Dissecting microscope
• 3 advantages
• 1 disadvantage

1. larger objects can be viewed (animal organs, fingers)
2. the images are 3D
3. the working distance is large allowing for easy manipulation of the specimen

1. low magnification
2. low resolution

25

Interphase
• 4

• rapid growth
• duplication of DNA
• DNA in form of chromatin
•Basic metabolic processes

26

Prophase
•6

• Chromatin thicken and shorten to form chromosomes
• Chromosomes are composed of 2 chromatids joined at the centromere
•nuclear membrane disappears
•nucleolus disappears
• Centrioles move to opposite poles
• spindle fibers form

27

Metaphase

Chromosomes line up along equator

28

Anaphase

Chromatids separate at centromere and are pulled to opposite pole by spindle fibers

29

Telophase
•5

•Chromosomes movements stop
• Chromosomes uncoil to form chromatin
• Nuclear membrane reforms
• Nucleolus reappears
• Spindle fibers disappear