Lab 3 Flashcards
(28 cards)
seed storage proteins vary from – of dry mass
10-40%
proteins act as – sinks
excess nitrogen
storage proteins are found in –
seeds, root and shoot tubers
mg protein per
g fresh weight of seed flour
use – to measure protein
dye-binding assay
BCA protein assay is a – formulation based on bicinchoninic acid for the colorimetric detection and quantization of the total protein
detergent-compatible
reduction of Cu+2 –> +1 by protein in a – medium
alkaline
albumins are – soluble
water
globulins dissolve in the presence of –
salt (0.4-1 M NaCl)
prolamins dissolve in –
short chain alcohols (ethanol and propanol)
glutelins dissolve in –solutions
alkaline (NaOH or KOH) or chaotropic agents (urea and guanidine HCl)
use a – to fine flour
coffee mill
put – g of seed flour in a test tube
0.2
albumins, globulins, – , gluteins
prolamins
1 ml H2O
albumins
1 ml of 1M NaCl then 1 ml H2O
globulins
70% isopropanol
prolamins
4 M urea
glutelins
each supernatant pool has a volume of at least
1ml
start with a – dilution with saline
fivefold
fivefold dilution is – sample + 4 ml saline
1 ml
a fivefold dilution means the addition of sufficient solvent to make the – five times the original
five times
0.1 ml sample + – working reagent
2 ml
blank control
2 ml WR + 0.1 ml water
