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Flashcards in Lab 3 Deck (28)
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1

seed storage proteins vary from -- of dry mass

10-40%

2

proteins act as -- sinks

excess nitrogen

3

storage proteins are found in --

seeds, root and shoot tubers

4

mg protein per

g fresh weight of seed flour

5

use -- to measure protein

dye-binding assay

6

BCA protein assay is a -- formulation based on bicinchoninic acid for the colorimetric detection and quantization of the total protein

detergent-compatible

7

reduction of Cu+2 --> +1 by protein in a -- medium

alkaline

8

albumins are -- soluble

water

9

globulins dissolve in the presence of --

salt (0.4-1 M NaCl)

10

prolamins dissolve in --

short chain alcohols (ethanol and propanol)

11

glutelins dissolve in --solutions

alkaline (NaOH or KOH) or chaotropic agents (urea and guanidine HCl)

12

use a -- to fine flour

coffee mill

13

put -- g of seed flour in a test tube

0.2

14

albumins, globulins, -- , gluteins

prolamins

15

1 ml H2O

albumins

16

1 ml of 1M NaCl then 1 ml H2O

globulins

17

70% isopropanol

prolamins

18

4 M urea

glutelins

19

each supernatant pool has a volume of at least

1ml

20

start with a -- dilution with saline

fivefold

21

fivefold dilution is -- sample + 4 ml saline

1 ml

22

a fivefold dilution means the addition of sufficient solvent to make the -- five times the original

five times

23

0.1 ml sample + -- working reagent

2 ml

24

blank control

2 ml WR + 0.1 ml water

25

incubate at

37 C for 30 min

26

read with spectrophotometer using plastic cuvettes at --

562 nm

27

BSA Bovine Albumin Standard determines the --

[albumin], [prolamin], [glutelin]

28

BGG Bovine Gamma Globulin

[globulin]