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Flashcards in Lab 8 Deck (37)
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1

weigh out -- spinach

3g

2

do not include -- for spinach

large vascular tissue

3

extract pigment should be bright green and -- ml

25

4

readings at 652 and 665 if you can't get good readings the extract may need to be diluted up to --

10 fold

5

gas pressure will arise in the stoppered funnel release pressure by inverting the funnel and then rotating until the upper layer contains all chlorophyll; turn the separatory funnel so the liquid is -- the stopper end then slowly turn the vent

towards

6

-- is polar enough to dissolve less hydrophobic pigments

aqueous methanol

7

separate chlorophyll and carotenoids of the petroleum ether layer by removing the -- from the chlorophyll (resulting chlorophyllide or green chromophore is soluble in water)

hydrophobic phytyl tail

8

-- is caustic (burn or corrode organic tissue)

KOH

9

vent the funnel -- the hood

towards

10

separate pigments in chlorophyll

thin layer chromatography

11

each chlorophyll pigment has a different polarity so it moves at a different rate up a TLC plate through -- when immersed in a solvent

capillary action

12

TLC: -- layer will be on top of chloroform and contains all the pigments in a concentrated form

hexane

13

TLC: remove hexane layer without --

aqueous methanol

14

hexane + pinch of -- to get a concentrated solution of the pigments

anhydrous NaSO4

15

allow solution to -- between each spotting and minimize light exposure

evaporate

16

keep TLC tank -- or the solvent will evaporate

covered

17

the level of petroleum ether: acetone must be -- than the spot on paer

lower

18

Rf = distance moved by -- / distance moved by --

pigment/solvent

19

slight differences in -- of plates and in the solvent composition will affect the Rf values

dryness

20

photosynthetic pigments are -- located within proteins in the lipoprotein membranes (thylakoids) of chloroplast

lipids

21

pigments can be extracted with -- that dissolve the membrane lipids

organic solvents

22

extracts = pigments involved in -- + colorless lipids of lipid bilayer

photosynthetic light-harvesting apparatus

23

molar ratio of a to b

3

24

molar ratio of chlorophyll to carotenoids

5

25

a and b have -- absorption spectra

similar

26

carotenoids absorb in -- region

blue

27

a have -- in the porphyrin ring while b has formyl group

methyl group

28

methyl group makes a -- than b in nonpolar solvents

more soluble

29

carotenoids have -- C atoms

40

30

carotenes (orange) are pure --

hydrocarbonds