Lab 4

Question 1

at cellular level, water transport is influenced by – and –

Answer 1

relative pressure and [solute] in water

Question 2

energy content of water expressed as –

Answer 2

water potential (pressure potential and solute potential)

Question 3

water potential is expressed in units of – and at 20C

Answer 3

pressure

Question 4

pressure potential in water solution is relative to the atmospheric pressure which is 1 bar (0.1 MPA) near –

Answer 4

sea level

Question 5

movement of water into cell does not increase in cell size but in –

Answer 5

pressure (turgor)

Question 6

cell solute potentials are –

Answer 6

-2.4 MPa

Question 7

dissolved solutes in water – energy or solute potential

Answer 7

decreases

Question 8

[solute] are expressed in terms of osmolarity which is the sum of – of all dissolved species in the solution

Answer 8

[molar]s

Question 9

1 M KCl solution in which KCl completely dissociates into K+ and Cl- ions = – osmolar solution

Answer 9

2

Question 10

water will move from the higher to lower water potential until an equilibrium situation is established in which the – inside and outside the cell are equal

Answer 10

solute potential

Question 11

plans usually have – internal pressure than their surrounding medium

Answer 11

higher (turgor)

Question 12

leaves which lose turgor due to water loss, show this lack of structural support by –

Answer 12

wilting

Question 13

at the cellular level, loss of turgor is seen as – caused the lack of internal pressure

Answer 13

gaps b/t plasma membrane and cell wall (plasmolysis)

Question 14

Cut – leaf into halves

Answer 14

Elodea

Question 15

place – pieces of tissue on microscope slides with 1-2 mL solution

Answer 15

2

Question 16

always ensure that the strips are –

Answer 16

completely submerged in solution

Question 17

in mesophyll cells, the thickly packed – are all contained in the thin layer of cytoplasm surrounding the vacuole and may be used as a marker for the withdraw of the plasma membrane

Answer 17

chloroplasts

Question 18

count out 30 cells in the right corner of the – and count how many are plasmolysed

Answer 18

field of view

Question 19

make a second measurement after 30 minutes of equilibrium (the first measurement was after 5 min) and be sure to count –

Answer 19

the same 30 cells as before

Question 20

T/F: you may need to interpolate the concentration

Answer 20

true

Question 21

disengaging the cell membrane from the cell wall is very stressful so it is more common to see –

Answer 21

cell shrinkage

Question 22

cell shrinks and cell membrane – taking the cell wall with it

Answer 22

withdraws

Question 23

cell collapse could have important effects on leaf hydraulic conduction which is capacity of water to move through the leaf and make up for –

Answer 23

water lost during transpiration when stomata open to capture CO2 for photosynthesis

Question 24

species for cell collapse

Answer 24

Eucalyptus and Lantana

Question 25

for each species take -- with two leaves each

Answer 25

2 branches

Question 26

two leaves from each species are to be dried and measured --

Answer 26

progressively

Question 27

the remaining two leaves should be put with petioles under water and be measured progressively and returned to water each time

Answer 27

control leaves

Question 28

before measuring the leaf mass,

Answer 28

dry the surface of the control leaes