Lab 7 Flashcards
(11 cards)
1
Q
endospore stain
A
differential stain
formed by some firmicutes
survival cells, form in response to starvation
2
Q
instructions
A
loop of water, add loop of microbe and mix
air dry and heat fix
stain
blot dry with bibulous paper
3
Q
primary stain
A
malachite green
added while on heat steam to keep moisturized, with paper towel on each circle
4
Q
endospores are
A
very difficult to stain so decolorizer step can be used for as long as you like
5
Q
mordant
A
heat steam for 15 min
6
Q
decolorizer
A
water for 1 min
7
Q
secondary stain
A
safranin for 1 min
8
Q
endospore color
A
green
9
Q
vegetative cell color
A
pink
10
Q
brain heart infusion agar
A
pink rod with blue-green endospore
11
Q
more endospores equals
A
fewer nutrients
