Lab 8 Flashcards
(11 cards)
1
Q
Acid-fast stain
Kinyoun stain
A
for mycobacteria
used to visualize acid-fast bacteria
2
Q
mycobacterium has
A
waxy mycolic acids in cell wall
repels stains
3
Q
primary stain
mordant
applied together, puddle 15 min
A
basic fuchsin
phenol (dissolve wax somewhat)
together: carbol fuchsin
4
Q
mordant adds color
A
red
5
Q
decolorizer
puddle, 10 sec
A
acid alcohol
6
Q
secondary stain
1 min
A
methylene blue
7
Q
Acid fast stains
A
red
8
Q
Non-fast stains
A
blue
9
Q
instructions
A
drop of H2O
mix loop of M. Seg
mix loop of S. aureus
air dry
heat fix
stain
10
Q
Mycobacterium smegmatis
A
BSL-1
acid-fast, found on healthy human skin and is rarely pathogenic
small pink bacilli
11
Q
Staphylococcus aureus
A
BSL-2
found on skin and in nose
blue cocci
