(LAB) ALP and ACP

Question 1

E.C. No. of ALP

Answer 1

E.C. 3.1.3.1

Question 2

Systematic Name of ALP

Answer 2

Orthophosphoric Monoester Phosphohydrolase (Alkaline Optimum)

Question 3

Isoenzyme ALP

Answer 3

Placental ALP
Bone ALP
Intestinal ALP
Liver ALP

Question 4

ALP Isoenzyme elevated in normal pregnancy

Answer 4

Placental ALP

Question 5

ALP Isoenzyme elevated in Blood type B and O individuals

Answer 5

Intestinal ALP

Question 6

ALP Isoenzyme elevated children or growing individuals

Answer 6

Bone ALP

Question 7

Other Isoenzymes of ALP (Abnormal Fractions)

Answer 7

Regan
Nagao
Kasahara

Question 8

Substrate of ALP

Answer 8

Phosphomonoester

Question 9

Product of ALP

Answer 9

Alcohol + Phosphate ion

Question 10

ALP Optimal pH

Answer 10

9.0 - 10.0

Question 11

ALP Activators

Answer 11

Mg2+ and Zn2+

Question 12

Most commonly used method Continuous-Monitoring Technique for ALP Assay

Answer 12

Bessey-Lowry and Brock method

Question 13

6 Methods of ALP Measurement

Answer 13

1. Shinowara
2. King and Armstrong
3. Bessey-Lowry and Brock
4. Huggins and Talalay
5. Moss
6. Klein, Babson, and Read

Question 14

Substrates of ALP Measurement Methods (In order based on table)

Answer 14

1. Beta-glycerophosphate
2. Phenyl Phosphate
3. Para-Nitrophenyl phosphate
4. Phenolphthalein Diphosphate
5. Alpha-Naphthol Phosphate
6. Buffered Phenolphthalein Phosphate

Question 15

End Products of ALP Measurement Methods (In order based on table)

Answer 15

1. Inorganic Phosphate + glycerol
2. Phenol
3. Para-Nitrophenol
4. Phenolphthalein red
5. Alpha-Naphthol
6. Free Phenolphthalein

Question 16

Acidic blood when measure ALP results

Answer 16

Falsely low

Question 17

ALP reference range for women

Answer 17

35 - 104 U/L

Question 18

ALP reference range for Men

Answer 18

40 -129 U/L

Question 19

ALP reference range for Children

Answer 19

54 - 369 U/L

Question 20

E.C. of ACP

Answer 20

E.C. 3.1.3.2

Question 21

Systemic Name of ACP

Answer 21

Orthophosphoric Monoester Phosphohydrolase (Acid Optimum)

Question 22

ACP Substrate

Answer 22

Phosphomonoester

Question 23

ASP Products

Answer 23

Alcohol & Phosphate Ion

Question 24

Substrate of choice for quantitative endpoint reaction

Answer 24

Thymolphthalein Monophosphate

Question 25

Substrate of choice for continuous monitoring methods

Answer 25

Alpha-Naphthyl Phosphate

Question 26

Richest Gland with ACP

Answer 26

Prostate Gland

Question 27

4 Methods of ACP Measurement

Answer 27

1. Gutman and Gutman 2. Shinowara 3. Bason, Read, and Philips 4. Ray and Hillman

Question 28

Substrates of ACP Measurement Methods (In order based on table)

Answer 28

1. Phenyl Phosphate 2. Para-Nitrophenyl Phosphate 3. Alpha-Naphthyl Phosphate 4. Thymolphthalein Monophosphate

Question 29

End Products of ACP Measurement Methods (In order based on table)

Answer 29

1. Inorganic Phosphate 2. Para-Nitrophenol 3. Alpha-Naphthyl 4. Free Thymolphthalein

Question 30

Substrate of Choice for Kinetic Method

Answer 30

Babson, Read, and Philips

Question 31

Substrate of Choice for End Point Reaction

Answer 31

Ray and Hillman

Question 32

Tissue sources of ACP that may cause false results

Answer 32

RBCs and Platelets

Question 33

When will ACP activity decrease when left at Room Temp

Answer 33

Within 1-2hrs

Question 34

Reference range of ACP for Men at 25C

Answer 34

3.6

Question 35

Reference range of ACP for Men at 30C

Answer 35

5.0

Question 36

Reference range of ACP for Men at 37C

Answer 36

6.5

Question 37

Reference range of ACP for Women at 25C

Answer 37

3.0

Question 38

Reference range of ACP for Women at 30C

Answer 38

4.2

Question 39

Reference range of ACP for Women at 37C

Answer 39

5.5