(LEC) Amylase & Lipase

Question 1

EC of Amylase

Answer 1

EC 3.2.1.1

Question 2

Other name for Amylase

Answer 2

1,4-D-Glucan Glucanohydrolase

Question 3

Molecular weight of Amylase

Answer 3

50-55 kDa

Question 4

Enzyme the catalyzes the breakdown of starch and glycogen

Answer 4

Hydrolase

Question 5

Consists of both Amylose & Amylopectin

Answer 5

Starch

Question 6

chain of glucose linked by a-1,4-glycosidic bond

Answer 6

Amylose

Question 7

chain of polysaccharide linked by a-1,6-glycosidic bond

Answer 7

Amylopectin

Question 8

Cofactors for Amylase

Answer 8

Ca2+ & Cl-

Question 9

Major Sources of Amylase

Answer 9

Salivary Glands
Pancreas

Question 10

Primary Isoenzymes of Amylase

Answer 10

S-isoamylase
P-isoamylase

Question 11

Reference Range of Serum Amylase

Answer 11

28-100 U/L

Question 12

Reference Range of Urine Amylase

Answer 12

1-15 U/h

Question 13

1 Somogyi Unit = ?

Answer 13

1.85 IU

Question 14

Clinical Correlation with Elevated Amylose Levels

Answer 14

Acute Pancreatitis
Salivary Gland Disease
Intra-abdominal Disease

Question 15

Condition when an amylase molecule combines with IgG or IgA or a polysaccharide to perform a complex that is too large to be filtered across the renal glomerulus

Answer 15

Macroamylasemia

Question 16

Amylase Isoenzyme that migrates quickly in Electrophoresis

Answer 16

S-amylase

Question 17

Amylase Isoenzyme that migrates slower in Electrophoresis

Answer 17

P-amylase

Question 18

Most commonly observed fractions in Amylase Electrophoresis

Answer 18

P2, S1, S2

Question 19

Predominant amylase isoenzyme in acute pancreatitis but not specific

Answer 19

P3

Question 20

Amylase Assay:

Measures the disappearance of starch substrate

Answer 20

Amyloclastic

Question 21

Amylase Assay:

Measures the appearance of the product

Answer 21

Saccharogenic

Question 22

Amylase Assay:
Measures increasing color from production of product coupled with a chromogenic dye

Answer 22

Chromogenic

Question 23

Amylase Assay:

Coupling of several enzyme systems to monitor amylase activity

Answer 23

Continuous Monitoring

Question 24

In Amyloclastic Method, ______ is attached to starch substrate forming dark blue color

Answer 24

Iodine

Question 25

Amylase Assay: Uses starch substrate that is hydrolyzed by amylase to its constituent carbohydrate molecules that have reducing properties

Answer 25

Saccharogenic Method

Question 26

Amylase Assay: Amylase hydrolyzes starch substrates, forming smaller water soluble dye substrate fragments resulting to increase in color intensity

Answer 26

Chromogenic Method

Question 27

Coupling Enzymes used in Continuous Monitoring Method

Answer 27

A-glucosidae Hexokinase G-6-P dehydrogenase

Question 28

Optimal pH in Continuous Monitoring Method

Answer 28

pH 6.9

Question 29

Preferentially inhibits salivary amylase

Answer 29

Wheat Germ Lectin

Question 30

Can suppress or inhibit amylase activity

Answer 30

Plasma Triglyceride

Question 31

Falsely elevates serum amylase

Answer 31

Morphine / Opioids

Question 32

Anticoagulant that should be used for Amylase Assays

Answer 32

Heparin

Question 33

EC of Lipase

Answer 33

EC 3.1.1.3

Question 34

Other name of Lipase

Answer 34

Triacylglycerol Acylhydrolase

Question 35

Molecular Weight of Lipase

Answer 35

45 kDa

Question 36

Major and Primary source of serum lipase

Answer 36

Pancreas

Question 37

Most clinically specific and sensitive Lipase Isoenzyme

Answer 37

L2

Question 38

Reference range for Serum Lipase

Answer 38

< 38 U/L

Question 39

Clinical Correlation of Elevated Lipase levels

Answer 39

Acute Pancreatitis Renal Disorder Humps virus

Question 40

Lipase Assay: Estimation of liberated fatty acids and turbidimetric method

Answer 40

Titrimetric method

Question 41

Substrate for Classic Cherry-Crandall Method

Answer 41

Olive Oil

Question 42

Substrate for Modified Cherry-Crandall Method

Answer 42

Triolein

Question 43

Lipase Assay: Based on coupled reaction with Coupling Enzymes such as - Glycerol Kinase - Glycerophosphate Oxidase - Peroxidase

Answer 43

Colorimetric Method

Question 44

Causes falsely low Lipase levels

Answer 44

Hemolysis